Pollen and pollen tube biology : methods and protocols /: methods and protocols. (2020)
- Record Type:
- Book
- Title:
- Pollen and pollen tube biology : methods and protocols /: methods and protocols. (2020)
- Main Title:
- Pollen and pollen tube biology : methods and protocols
- Further Information:
- Note: Edited by Anja Geitmann.
- Editors:
- Geitmann, Anja
- Contents:
- Preface… Table of Contents… Contributing Authors… 1. Pollen Grain Counting Using a Cell Counter Hiroyuki Kakui, Misako Yamazaki, Naoto-Benjamin Hamaya, and Kentaro K. Shimizu 2. A Toolkit for teasing Apart the Early Stages of Pollen-Stigma Interactions in Arabidopsis thaliana Hyun Kyung Lee, Stuart Macgregor, and Daphne R. Goring 3. Decapitation Crosses to Test Pollen Fertility Mutations for Defects in Stigma-Style Penetration Chrystle Weigand and Jeffrey Harper 4. Isolation of the Pistil-Stimulated Pollen Tube Secretome Said Hafidh and David Honys 5. Restricted Pollination for Tracing Individual Pollen Tubes in a Pistil Taro Takahashi, Toshiyuki Mori, and Tomoko Igawa 6. Semi-In Vivo Assay for Pollen Tube Attraction Sheng Zhong, Zhijuan Wang, and Li-Jia Qu 7. Isolation and Cloning of Suppressor Mutants with Improved Pollen Fertility Steven Beuder and Cora A. MacAlister 8. Workflow to Characterize Mutants with Reproductive Defects Jennifer A. Noble and Ravishankar Palanivelu 9. Internally-Controlled Methods to Quantify Pollen Tube Growth and Penetration Defects in Arabidopsis thaliana Devin K. Smith and Ian S. Wallace 10. Arabidopsis thaliana Pollen Tube Culture for Multi-Omics Studies M. Costa, J. Silva, and S. Coimbra 11. Large Scale Analysis of Pollen Viability and Oxidative Level using H2DCFDA-Staining Coupled with Flow Cytometry Nicholas Rutley and Gad Miller 12. Obtaining Mutant Pollen for Phenotypic Analysis and Pollen Tube Dual-Staining Sheng Zhong, Zhijuan Wang, andPreface… Table of Contents… Contributing Authors… 1. Pollen Grain Counting Using a Cell Counter Hiroyuki Kakui, Misako Yamazaki, Naoto-Benjamin Hamaya, and Kentaro K. Shimizu 2. A Toolkit for teasing Apart the Early Stages of Pollen-Stigma Interactions in Arabidopsis thaliana Hyun Kyung Lee, Stuart Macgregor, and Daphne R. Goring 3. Decapitation Crosses to Test Pollen Fertility Mutations for Defects in Stigma-Style Penetration Chrystle Weigand and Jeffrey Harper 4. Isolation of the Pistil-Stimulated Pollen Tube Secretome Said Hafidh and David Honys 5. Restricted Pollination for Tracing Individual Pollen Tubes in a Pistil Taro Takahashi, Toshiyuki Mori, and Tomoko Igawa 6. Semi-In Vivo Assay for Pollen Tube Attraction Sheng Zhong, Zhijuan Wang, and Li-Jia Qu 7. Isolation and Cloning of Suppressor Mutants with Improved Pollen Fertility Steven Beuder and Cora A. MacAlister 8. Workflow to Characterize Mutants with Reproductive Defects Jennifer A. Noble and Ravishankar Palanivelu 9. Internally-Controlled Methods to Quantify Pollen Tube Growth and Penetration Defects in Arabidopsis thaliana Devin K. Smith and Ian S. Wallace 10. Arabidopsis thaliana Pollen Tube Culture for Multi-Omics Studies M. Costa, J. Silva, and S. Coimbra 11. Large Scale Analysis of Pollen Viability and Oxidative Level using H2DCFDA-Staining Coupled with Flow Cytometry Nicholas Rutley and Gad Miller 12. Obtaining Mutant Pollen for Phenotypic Analysis and Pollen Tube Dual-Staining Sheng Zhong, Zhijuan Wang, and Li-Jia Qu 13. Galvanotropic Chamber for Controlled Reorientation of Pollen Tube Growth and Simultaneous Confocal Imaging of Intracellular Dynamics Firas Bou Daher and Anja Geitmann 14. Analyzing Intracellular Gradients in Pollen Tubes Daniel S. C. Damineli, Maria Teresa Portes, and José A. Feijó 15. Silicone Chambers for Pollen Tube Imaging in Microstructures In Vitro Environments Hana Bertrand-Rakusová, Youssef Chebli, and Anja Geitmann 16. Analyzing the Impact of Protein Overexpression on Ca2+ Dynamics and Development in Tobacco Pollen Tubes Chunxia Zhang, Leonie Steinhorst, and Jörg Kudla 17. Imaging and Analysis of the Content of Callose, Pectin, and Cellulose in the Cell Wall of Arabidopsis Pollen Tubes Grown In Vitro Ana R. Sede, Diego L. Wengier, Cecilia Borassi, José M. Estevez, and Jorge P. Muschietti 18. Characterization of Growth Behavior and the Resulting Forces Applied by Pollen Tubes in a 3D Matrix Ronny Reimann and Delf Kah 19. Flow Chamber Assay to Image the Response of FRET-Based Nanosensors in Pollen Tubes to Changes in Medium Composition Theresa Maria Reimann 20. Quantification of Mechanical Forces and Physiological Processes Involved in Pollen Tube Growth Using Microfluidics and Microrobotics Jan Burri, Gautam Munglani, Bradley J. Nelson, Ueli Grossniklaus, and Hannes Vogler 21. Measuring Exocytosis Rate in Arabidopsis Pollen Tube Using Corrected Fluorescence Recovery after Photoconversion (cFRAPc) Technique Jingzhe Guo and Zhenbiao Yang 22. Testing Pollen Tube Proteins for In Vivo Binding to Phosphatidic Acid by n0Butanol Treatment and Confocal Microscopy Carolin Fritz and Benedikt Kost Subject Index List…. … (more)
- Publisher Details:
- New York, NY : Humana Press
- Publication Date:
- 2020
- Extent:
- 1 online resource (xiii, 332 pages), illustrations (some color)
- Subjects:
- 571.8/452
Pollen -- Laboratory manuals
Electronic books - Languages:
- English
- ISBNs:
- 9781071606728
1071606727 - Related ISBNs:
- 9781071606711
- Notes:
- Note: Online resource; title from PDF title page (SpringerLink, viewed July 1, 2020).
- Access Rights:
- Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
- Access Usage:
- Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.
- View Content:
- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library HMNTS - ELD.DS.513123
- Ingest File:
- 03_094.xml