Axon degeneration : methods and protocols /: methods and protocols. (2020)

• Record Type: Book
• Further Information: Note: Edited by Elisabetta Babetto.
• Editors: Babetto, Elisabetta
• Contents: Part I: Introduction 1. Axon Degeneration: Which Method to Choose? Michael P. Coleman Part II: In Vitro Assays 2. Axon Degeneration Assays in Superior Cervical Ganglion Explant Cultures Andrea Loreto and Jonathan Gilley 3. Microinjection of Superior Cervical Ganglion Neurons for Studying Axon Degeneration Jonathan Gilley and Andrea Loreto 4. Assessing Axonal Degeneration in Embryonic Dorsal Root Ganglion Neurons In Vitro Jung Eun Shin and Yongcheol Cho 5. Viral Transduction of DRG Neurons Yo Sasaki 6. Planning and Analysis of Axon Degeneration Screening Experiments Lyndah Lovell, Jack Bramley, and William Buchser 7. A Microfluidic Culture Platform to Assess Axon Degeneration Yu Yong, Christopher Hughes, and Christopher Deppmann 8. A Schwann Cell-Neuron Co-Culture System to Study Neuron-Glia Interaction during Axon Degeneration Elisabetta Babetto 9. Establishing Myelinating Co-Cultures Using Human iPSC-Derived Sensory Neurons to Investigate Axonal Degeneration and Demyelination Alex Clark Part III: Ex Vivo Assays 10. Preparation of Organotypic Hippocampal Slice Cultures for the Study of CNS Disease and Damage Claire S. Durrant 11. Organotypic Culture Assay for Neuromuscular Synaptic Degeneration and Function Kosala N. Dissanayake, Robert Chang-Chih Chou, Rosalind Brown, and Richard R. Ribchester 12. Intracellular Recordings of Postsynaptic Voltage Responses at the Drosophila Neuromuscular Junction Vera Valakh and Johanna G. Flyer-Adams 13. Ex Vivo Studies of Optic Nerve Axon Electrophysiology Chinthasagar Bastian, Sylvain Brunet, and Selva Baltan 14. Ex Vivo Analysis of Axonal Degeneration Using Sciatic and Optic Nerve Preparations Rodrigo López-Leal and Felipe A. Court 15. Measuring Bioenergetic Signatures of Peripheral Nerve Segments by Extracellular Flux Analysis Bogdan Beirowski Part IV: In Vivo Assays 16. Detection of Neutrophils in the Sciatic Nerve Following Peripheral Nerve Injury Jon P. Niemi, Jane A. Lindborg, and Richard E. Zigmond 17. Whole-Tissue Immunolabeling and 3D Fluorescence Imaging to Visualize Axon Degeneration in the Intact, Unsectioned Mouse Tissues Ying Cao and Jing Yang 18. Transmission Electron Microscopy and Morphometry of the CNS White Matter Julia M. Edgar, Rebecca Sherrard Smith, and Ian D. Duncan 19. In Vivo Calcium Imaging During Axon Degeneration in Zebrafish Mauricio E. Vargas 20. In Vivo Imaging of Anterograde and Retrograde Axonal Transport in Rodent Peripheral Nerves James N. Sleigh, Andrew P. Tosolini, and Giampietro Schiavo 21. In Vivo Visualization of Moving Synaptic Cargo Complexes within Drosophila Larval Segmental Axons Rupkatha Banerjee, Joseph A. White II, and Shermali Gunawardena 22. A Chemotherapy-Induced Peripheral Neuropathy Model in Drosophila melanogaster Martha R.C. Bhattacharya 23. Models of Axon Degeneration in Drosophila Larvae E.J. Brace and Aaron DiAntonio 24. In Vivo Analysis of Glial Immune Responses to Axon Degeneration in Drosophila melanogaster Mary A. Logan and Sean D. Speese.
• Publisher Details: New York, NY : Humana Press
• Publication Date: 2020
• Extent: 1 online resource (xi, 340 pages), illustrations (some color)
• Subjects: 612.8/1046; Axons -- Laboratory manuals; Nervous system -- Degeneration -- Laboratory manuals; Electronic books
• Languages: English
• ISBNs: 9781071605851; 1071605852
• Related ISBNs: 9781071605844; 1071605844
• Notes: Note: Online resource; title from PDF title page (SpringerLink, viewed July 1, 2020).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.