Artificial assemblies with cooperative DNA recognition. (2020)

• Record Type: Book
• Further Information: Note: Zutao YU.
• Other Names: YU, Zutao
• Contents: Intro -- Supervisor's Foreword -- Part of this thesis has been published in the following journal articles: -- Acknowledgements -- Contents -- 1 Synthetic DNA Binding Assembly: Architecture, Application and Perspectives -- 1.1 Introduction -- 1.2 Structural Domains of Natural Transcription Factors (TFs) -- 1.2.1 DNA Binding Domain (DBD) -- 1.2.2 Cooperative Interaction Domain (CID) -- 1.2.3 Functional Domain (FD) -- 1.3 Synthetic DBD -- 1.3.1 Natural Product -- 1.3.2 Pyrrole-Imidazole Polyamides (PIPs) -- 1.3.3 Nucleic Acid-Based DNA Binding Domain -- 1.4 Synthetic CID 1.4.1 Nucleic Acid-Based CID -- 1.4.2 Metal Ion -- 1.4.3 Host-Guest System -- 1.4.4 Other Systems -- 1.5 Synthetic FD -- 1.5.1 Synthetic Enzymatic Modulators -- 1.5.2 Others -- 1.6 Artificial Assembly of DBD, CID and FD -- 1.6.1 Application of DBD -- 1.6.2 DBD + FD -- 1.6.3 DBD + CID -- 1.6.4 DBD + FD + CID -- 1.7 Perspectives -- References -- 2 PIP-HoGu, an Artificial Assembly with Cooperative DNA Recognition -- 2.1 Research Background -- 2.2 Design of PIP-HoGu System -- 2.3 In Vitro Evaluation of PIP-HoGu System -- 2.4 Cell Study of PIP-HoGu System -- 2.5 Conclusions 3.2.6 The Effect of PNA Length and Conjugation Site on Cooperative Binding -- 3.2.7 Competitive Assay -- 3.3 Conclusions -- 3.4 Supporting Information -- 3.4.1 Materials and Methods -- 3.4.2 Supporting Tables and Figures -- 3.4.3 MS and HPLC Data -- References -- 4 ePIP-HoGu, A Cooperative DNA Binding System to Recruit Epigenetic Modifier to the Targeted DNA Locus -- 4.1 Research Background -- 4.2 Design of Second-Generation PiP-HoGu System by Introducing CB7 -- 4.3 Characterization of CB7-Based PIP-HoGu System -- 4.4 ePIP-HoGu Cooperatively Acetylate Specific Targeted Histones -- 4.5 Conclusions 4.6 Supporting Information -- 4.6.1 Materials and Methods -- 4.6.2 Supporting Tables and Figures -- 4.6.3 MS and HPLC Data -- References -- Curriculum Vitae -- Education -- Awards and Honors -- Research Statement and Interests
• Publisher Details: Singapore : Springer
• Publication Date: 2020
• Extent: 1 online resource (145 p.)
• Subjects: 572.8/6; DNA-binding proteins; Synthetic biology; DNA-binding proteins; Synthetic biology; Electronic books; Electronic books
• Languages: English
• ISBNs: 9789811544231; 9811544239
• Related ISBNs: 9811544220; 9789811544224
• Notes: Note: Includes bibliographical references.
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.