Study on the cellular regulation and function of lysine malonylation, glutarylation and crotonylation. (2020)

• Record Type: Book
• Further Information: Note: Xiucong Bao.
• Other Names: Bao, Xiucong
• Contents: Intro -- Supervisor's Foreword -- Parts of this thesis have been published in the following journal articles: -- Acknowledgements -- Contents -- Abbreviations -- 1 Introduction to Protein Posttranslational Modifications (PTMs) -- 1.1 Protein Posttranslational Modifications -- 1.2 Regulation of Chromatin Dynamics by Histone Modifications -- 1.3 Lysine Acetylation, Succinylation, Malonylation, Glutarylation and Crotonylation -- 1.3.1 Lysine Acetylation -- 1.3.2 Lysine Succinylation -- 1.3.3 Lysine Malonylation -- 1.3.4 Lysine Glutarylation -- 1.3.5 Lysine Crotonylation 1.4 Approaches for Identification of Protein Substrates of Specific Modifications -- 1.4.1 Chemical Affinity Enrichment -- 1.4.2 Antibody Based Immunoaffinity Enrichment -- 1.4.3 Chemical Reporters for Protein Posttranslational Modifications -- 1.5 Identification of Protein-Protein Interactions -- 1.5.1 Yeast 2-Hybrid System -- 1.5.2 Protein Affinity Chromatography -- 1.5.3 Co-immunoprecipitation (Co-IP) -- 1.5.4 Photo-Cross-Linking -- 1.6 The Objectives and Organization of This Thesis -- References -- 2 Chemical Reporter for Lysine Malonylation -- 2.1 Introduction 2.2 Design of the First-Generation Chemical Reporter, Mal-yne, for Kmal -- 2.3 Labeling of Cellular Proteins by Mal-yne -- 2.4 Design of the Second-Generation Chemical Reporter, MalAM-yne, for Kmal -- 2.5 Comparison Between Mal-yne and MalAM-yne -- 2.6 Monitoring Dynamics of Lysine Malonylation -- 2.7 Sirt5 Regulates Lysine Malonylation -- 2.8 Evolutionary Conservation of Lysine Malonylation -- 2.9 Immunoblotting Analysis of Known Malonylated Proteins -- 2.10 Profiling of Malonylated Proteins Using MalAM-yne -- 2.11 Immunoblotting Analysis of Candidates of Lysine Malonylation 2.12 Detection of Mal-yne Modified Lysine Sites -- 2.13 Functional Annotation of Lysine Malonylomes -- 2.14 Discussion -- 2.15 Experimental Methods -- 2.15.1 Cell Culture -- 2.15.2 Metabolic Labeling and Preparation of Cell Lysates -- 2.15.3 Cu(I)-Catalyzed Cycloaddition/Click Chemistry -- 2.15.4 In-gel Fluorescence Visualization -- 2.15.5 Streptavidin Affinity Enrichment of Biotinylated Proteins -- 2.15.6 Sample Preparation for Mass Spectrometry -- 2.15.7 Mass Spectrometry -- 2.15.8 Immunoblotting -- References -- 3 Identification of Histone Lysine Glutarylation -- 3.1 Introduction 3.2 Design of Chemical Reporter, GluAM-yne, for Lysine Glutarylation -- 3.3 Metabolic Labeling of Cellular Proteins by GluAM-yne -- 3.4 Metabolic Labeling of Human Histones by GluAM-yne -- 3.5 Identification of Histone Lysine Glutarylation -- 3.6 Validation of Histone Lysine Glutarylation -- 3.7 Deglutarylation Activity of Sirt5 -- 3.8 Discussion -- 3.9 Experimental Methods -- 3.9.1 Cell Culture -- 3.9.2 Metabolic Labeling and Preparation of Cell Lysates -- 3.9.3 Cu(I)-Catalyzed Cycloaddition/Click Chemistry -- 3.9.4 In-gel Fluorescence Visualization -- 3.9.5 Immunoblotting
• Publisher Details: Singapore : Springer
• Publication Date: 2020
• Extent: 1 online resource (177 pages)
• Subjects: 572/.6; Lysine; Lysine; Electronic books
• Languages: English
• ISBNs: 9789811525094; 9811525099; 9789811525100; 9811525102; 9789811525117; 9811525110
• Related ISBNs: 9789811525087; 9811525080
• Notes: Note: Includes bibliographical references.; Note: Print version record.
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.