Zika virus : methods and protocols /: methods and protocols. (2020)

• Record Type: Book
• Further Information: Note: Edited by Gary Kobinger, Trina Racine.
• Editors: Kobinger, Gary; Racine, Trina
• Contents: Zika Virus: A Brief History and Review of its Pathogenesis Rediscovered -- Zika Virus Isolation, Purification, and Titration -- Evaluating Zika Virus Pathogenesis in Immunocompromised Mice -- Droplet Digital PCR and Immunohistochemistry Techniques to Detect Zika Virus in the Central Nervous System of Mice -- A Plaque Reduction Neutralization Test for the Detection of ZIKV-Specific Antibodies -- Analysis of Serum Anti-Zika Virus Antibodies by Focus Reduction Neutralization Test -- Enzyme-Linked Immunosorbent Assay and Quantitative Reverse Transcription PCR as a Technique to Analyze Inflammation -- Production and Purification of Zika Virus NS1 Glycoprotein in HEK293 Cells -- Optimization of Small-Scale Production of Zika Virus Envelope Glycoprotein by Transient Expression in HEK293 Cells for ELISA -- Zika Virus Peptide ELISA (ZIKV-NS2B-concat ELISA) for Detection of IgG Antibodies to Zika Virus Infection -- Zika Virus Amplification using Strand Displacement Isothermal Method and Sequencing using Nanopore Technology -- Detection of Zika Virus using Reverse Transcription-Loop-Mediated Isothermal Amplification (RT-LAMP) -- Development and Validation of Real-Time RT-LAMP Assays for the Specific Detection of Zika Virus -- Comparative Analysis of Zika Virus Detection by RT-qPCR, RT-LAMP, and RT-RPA -- A Porcine Model of Zika Virus Infection to Profile the In Utero Interferon Alpha Response -- Isolation and Detection of Zika Virus-Infected Rhesus Macaques Lymph Node Cells and Splenocytes -- Discovery of Zika Virus Dependency and Restriction Factors using Flow-Based Arrayed CRISPR Screening for Identification of Targets (FACS-IT) -- Zika Virus Targets Multiple Tissues and Cell Types During the First Trimester of Pregnancy -- Use of Primary Human Fetal Astrocytes and Tissue Explants as Ex Vivo Models to Study Zika Virus Infection of the Developing Brain -- Isolation of Monoclonal Antibodies from Zika Virus-Infected Patient Samples.
• Publisher Details: New York : Humana
• Publication Date: 2020
• Copyright Date: 2020
• Extent: 1 online resource (290 pages)
• Subjects: 616.9/185; Zika virus; Zika Virus; Zika virus
• Languages: English
• ISBNs: 9781071605813
• Related ISBNs: 9781071605806
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.