Clinical and preclinical models for maximizing healthspan : methods and protocols /: methods and protocols. ([2020])

• Record Type: Book
• Further Information: Note: Edited by Paul C. Guest.
• Editors: Guest, Paul C
• Contents: Part I: Reviews 1. The Impact of New Biomarkers and Drug Targets on Age-Related Disorders Paul C. Guest 2. Vitamin D and Muscle Sarcopenia in Aging Behnaz Abiri and Mohammadreza Vafa 3. Quantitative Assays of Plasma Apolipoproteins Anne Poljak, Mark W. Duncan, Tharusha Jayasena, and Perminder S. Sachdev 4. Protocol for the Use of the Ketogenic Diet in Preclinical and Clinical Practice Ann-Katrin Kraeuter, Paul C. Guest, and Zoltan Sarnyai Part II: Protocols 5. An In Vivo/Ex Vivo Study Design to Investigate Effects of Chronic Conditions and Therapeutic Compounds on Adipose Stem Cells in Animal Models Hanél Sadie-Van Gijsen, Liske Kotzé-Hörstmann, and Barbara Huisamen 6. Model for Studying the Effects of Chronic Metabolic Disease on Endogenous Bone Marrow Stem Cell Populations Yashar Mehrbani Azar, Maria Jacoba Kruger, Dalene de Swardt, Michelle Maartens, Ascentia Mathapelo Seboko, William Frank Ferris, and Mari van de Vyver 7. Investigating Alcohol Behavior and Physiology Using Drosophila Melanogaster Aliza K. De Nobrega, Kristine V. Luz, Katherine N. Lyons, and Lisa C. Lyons 8. Using Genome-Editing Tools to Develop a Novel In Situ Coincidence Reporter Assay for Screening ATAD3A Transcript Inhibitors Liwei Lang and Yong Teng 9. Visualizing and Evaluating Cancer Cell Growth and Invasion by a Novel 3D Culture System Chloe Shay, Liwei Lang, and Yong Teng 10. Exploiting Plug-and-Play Electrochemical Biosensors to Determine the Role of FGF19 in Sorafenib-Mediated Superoxide and Nitric Oxide Production in Hepatocellular Carcinoma Cells Lixia Gao and Yong Teng 11. Proteomic Analysis of the Anoikis-Resistant Human Breast Cancer Cell Lines Chareeporn Akekawatchai, Sittiruk Roytrakul, Narumon Phaonakrop, Janthima Jaresitthikunchai, and Sarawut Jitrapakdee 12. Real-Time PCR Analysis of Metabolism-Related Genes in a Long-Lived Model of C. Elegans Sumino Yanase 13. Clinical Assessment of the NADome as Biomarkers for Healthy Aging Tharusha Jayasena, Sonia Bustamante, James Clement, Robert Welschinger, Gideon A. Chaplan, Perminder S. Sachdev, and Nady Braidy 14. 2D Gel Electrophoresis Combined with Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Analysis of Eye Lens to Identify Biomarkers of Age-Related Cataract Paul C. Guest 15. Testing the Effects of Dietary Seafood Consumption on Depressive Symptoms Maximus Berger, G. Paul Amminger, Robyn McDermott, Paul C. Guest, and Zoltán Sarnyai 16. Assays for Monitoring the Effects of Nicotinamide Supplementation on Mitochondrial Activity in Saccharomyces Cerevisiae Ivan Orlandi and Marina Vai 17. Measurement of a Surrogate Biomarker for Arginine Vasopressin Secretion is Association with Physiometric and Molecular Biomarkers of Aging Paul C. Guest, Hans-Gert Bernstein, Henrik Dobrowolny, Katrin Borucki, Sabine Westphal, and Johann Steiner 18. Liquid Chromatography Tandem Mass Spectrometry Analysis of Proteins Associated with Age-Related Disorders in Human Pituitary Tissue Paul C. Guest and Daniel Martins de-Souza 19. Coenzyme Q10 Assessment and the Establishment of a Neuronal Cell Model of CoQ10 Deficiency Robert Heaton, Kate Duberley, and Iain P. Hargreaves 20. Analyzing Mitochondrial Function in Brown Adipocytes with a Bioenergetic Analyzer Justin Darcy, Chih-Hao Wang, and Yu-Hua Tseng 21. Quantitative Analysis of DNA Methylation by Bisulfite Sequencing Vasily V. Ashapkin, Lyudmila I. Kutueva, and Boris F. Vanyushin 22. Histomorphometric Analysis of Anti-Aging Properties on Rat Skin Idha Kusumawati, Kresma Oky Kurniawan, Subhan Rullyansyah, and Eka Pramyrtha Hestianah 23. Integration of qRT-PCR and Immunohistochemical Techniques for mRNA Expression and Localization of m1AChR in the Brain of Aging Rat S. Asha Devi and S. Abhijit 24. Randomized Study Design to Test Effects of Vitamin D and Omega-3 Fatty Acid Supplementation as Adjuvant Therapy in Colorectal Cancer Patients Fatemeh Haidari, Behnaz Abiri, Masood Iravani, Kambiz Ahmadi-Angali, and Mohammadreza Vafa 25. Effect of Vitamin D Supplementation on Muscle Strength, Muscle Function, and Body Composition in Vitamin D-Deficient Middle-Aged Women Behnaz Abiri, Mohsen Dehghani, and Mohammadreza Vafa 26. The Association of Food Intake and Physical Activity with Body Composition, Muscle Strength, and Muscle Function in Postmenopausal Women Mohammadreza Vafa, Behnaz Abiri, and Mohsen Dehghani 27. Absolute Quantification of Plasma Apolipoproteins for Cardiovascular Disease Risk Prediction Betul Ozdemir, Zeliha Selamoglu, and Nady Braidy 28. Multiplex Analysis of Circulating Hormone Levels in Rat Models of Age-Related Diseases Paul C. Guest 29. Proteomic Analysis of Brain Tissue from a Chronic Model of Stress Using a Combined 2D Gel Electrophoresis and Mass Spectrometry Approach Paul C. Guest 30. Proteomic Analysis of Rat Hippocampus for Studies of Cognition and Memory Loss with Aging Paul C. Guest, Hassan Rahmoune, and Daniel Martins-de Souza 31. Brain Proteomic Analysis on the Effects of the Antidepressant Fluoxetine Paul C. Guest 32. MK-801 Treatment of Oligodendrocytes as a Cellular Model of Aging Paul C. Guest.
• Publisher Details: New York, N.Y : Humana Press
• Publication Date: 2020
• Extent: 1 online resource (xiv, 451 pages), illustrations
• Subjects: 612.6/7; Aging -- Prevention; Age factors in disease; Aging; Age Factors; Longevity; Models, Animal; Biochemistry; Pharmaceutical technology; Medical research; Science -- Life Sciences -- Biochemistry; Medical -- Pharmacy; Science -- Life Sciences -- Biology -- Molecular Biology; Age factors in disease; Aging -- Prevention; Laboratory Manual
• Languages: English
• ISBNs: 9781071604717; 1071604716
• Related ISBNs: 1071604708; 9781071604700
• Notes: Note: Includes bibliographical references and index.; Note: Online resource; title from PDF title page (SpringerLink, viewed May 10, 2021).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.