Innate lymphoid cells : methods and protocols /: methods and protocols. (2020)

• Record Type: Book
• Further Information: Note: Edited by Shoba Amarnath.
• Editors: Amarnath, Shoba
• Contents: Protocols for Innate Lymphoid Cell Phenotypic and Functional Characterization: An Overview -- Protocols for Studying Murine ILC Development -- Identification of Murine Innate Lymphoid Cell Subsets in Barrier Tissues and their Draining Lymph Nodes using Flow Cytometry -- Identification and Functional Characterization of Murine Group 3 Innate Lymphoid Cell (ILC3) Subsets in the Intestinal Tract and Associated Lymphoid Tissues -- Identification of Murine and Human Innate Lymphoid Cells in Frozen Tissue Sections using Immunofluorescence -- Assessing Phosphorylation of STAT Transcription Factors in Mouse Innate Lymphoid Cells -- Determining Co-Receptor Expression and Function in Murine ILC2 through Flow Cytometry Characterization and Co-Culture Techniques -- Protocol for Determining the Effect of Neuroendocrine Hormones on Murine ILC Function -- Role of G-Protein Coupled Receptors in Chemotaxis of Innate Lymphoid Cells -- Group 2 ILC Functional Assays in Allergic Airway Inflammation -- Isolation, Culture, and Induction of Plasticity in ILC2s -- Bacterial Infection Allows for Functional Examination of Adoptively Transferred Mouse Innate Lymphoid Cell Subsets -- Isolation, Characterization, and Culture of Intestinal Intraepithelial Lymphocytes -- Isolation and Characterization of Innate Lymphoid Cells within the Murine Tumor Microenvironment -- Microbiota Analysis using Sequencing by Synthesis: From Library Preparation to Sequencing -- Establishing Human Intestinal Enteroid/Organoid Lines from Preterm Infant and Adult Tissue -- Characterizing Innate Lymphoid Cell Phenotype and Function in Human Inflammatory Bowel Disease -- CRISPR/Cas9-Based Gene Engineering of Human Natural Killer Cells: Protocols for Knock-Out and Read-Outs to Evaluate the Efficacy.
• Publisher Details: New York, NY : Humana Press
• Publication Date: 2020
• Extent: 1 online resource (xiii, 242 pages), illustrations (some color)
• Subjects: 614.5999; Cancer -- Research; Immunology; Cytology; Lymphocytes; Immunity, Innate; Cancer -- Research; Cytology; Immunology; Laboratory Manual
• Languages: English
• ISBNs: 9781071603383; 1071603388
• Related ISBNs: 9781071603376
• Notes: Note: Includes bibliographical references and index.
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.