Characterization of plant viruses : methods and protocols /: methods and protocols. ([2020])

• Record Type: Book
• Further Information: Note: Alangar Ishwara Bhat, Govind Pratap Rao.
• Authors: Bhat, A. I; Rao, G. P (Govind P.)
• Contents: Chapter 1. Glasshouse for maintenance of virus and insect culture -- Chapter 2. Symptoms of virus infected plants -- Chapter 3. Isolation and diagnosis of virus through indicator hosts -- Chapter 4. Host range of viruses -- Chapter 5. Physico-chemical properties of virus in crude sap -- Chapter 6. Mechanical sap transmission -- Chapter 7. Transmission through grafting and budding -- Chapter 8. Transmission through dodder -- Chapter 9. Virus transmission through Pollen -- Chapter 10. Transmission through seeds -- Chapter 11. Transmission of viruses by aphids -- Chapter 12. Transmission of viruses by leafhoppers -- Chapter 13. Transmission of virusesby whiteflies -- Chapter 14. Transmission of virusesby thrips -- Chapter 15. Transmission of viruses through mealybugs -- Chapter 16. Transmission of viruses through beetles -- Chapter 17. Transmission of viruses through mites -- Chapter 18. Transmission of viruses through fungi -- Chapter 19. Transmission of viruses through nematodes -- Chapter 20. Storage and preservation of plant virus cultures -- Chapter 21. Purification of Plant Viruses -- Chapter 22. Ultraviolet absorption spectra of purified virus preparation -- Chapter 23. Electron microscopy and Utramicrotomy -- Chapter 24. Determination of coat protein molecular weight of viruses -- Chapter 25. Isolation of nucleic acid from purified virus and determination of its nature -- Chapter 26. Agarose gel electrophoresis for nucleic acids -- chapter 27. In vitro expression of viral coat protein in prokaryotic system and its purification -- Chapter 28. Production of Polyclonal Antiserum -- Chapter 29. Production of monoclonal antibody -- chapter 30. Serological Tests -- Chapter 31. Isolation of total DNA from plants -- Chapter 32. Isolation of total RNA from plants -- Chapter 33. Isolation of double stranded (ds) RNA from virus infected plants -- Chapter 34. Dot-blot hybridization technique -- Chapter 35. Polymerase chain reaction -- Chapter 36. Real-time polymerase chain reaction -- Chapter 37. DNA Microarray for detection of plant viruses -- Chapter 38. Loop-mediated isothermal amplification (LAMP) -- Chapter 39. Rolling circle amplification (RCA) -- Chapter 40. Recombinase polymerase amplification -- Chapter 41. Next generation sequencing for diagnosis of viruses -- Chapter 42. Cloning of PCR Product -- Chapter 43. cDNA synthesis and cloning -- Chapter 44. DNA sequencing -- Chapter 45. Sequence analysis and phylogenetic studies -- Chapter 46. Development of infectious clone of virus -- Chapter 47. Virus elimination by meristem-tip culture -- Chapter 48. Virus elimination through somatic embryogenesis -- Chapter 49. Production of virus-resistant plants through transgenic approaches -- Chapter 50. Production of virus-resistant plants through CRISPR-Cas technology.
• Publisher Details: New York, NY : Humana Press
• Publication Date: 2020
• Extent: 1 online resource (xxi, 552 pages), illustrations, portrait
• Subjects: 571.92; Plant diseases; Virology; Plant genetics; Plant physiology; Plant anatomy; Plants -- Development; Plant Viruses; Plant anatomy; Plant diseases; Plant genetics; Plant physiology; Plants -- Development; Virology; Electronic books; Laboratory Manual
• Languages: English
• ISBNs: 9781071603345; 1071603345; 9781071603338; 1071603337
• Notes: Note: Includes bibliographical references.; Note: Online resource; title from PDF title page (SpringerLink, viewed January 25, 2021).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.