Expression, purification, and structure biology of membrane proteins. (2020)

• Record Type: Book
• Further Information: Note: Edited by Camilo Perez and Timm Maier.
• Editors: Perez, Camilo; Maier, Timm
• Contents: 1. Cloning and Multi-Subunit Expression of Mitochondrial Membrane Protein Complexes in Saccharomyces cerevisiae Porsha L.R. Shaw, Kathryn A. Diederichs, Ashley Pitt, Sarah E. Rollauer, and Susan K. Buchanan 2. Membrane Protein Production in Escherichia coli Benjamin C. McIlwain and Ali A. Kermani 3. Membrane Protein Production in Lactococcus Lactis for Structural Studies Chloe Martens 4. Expression and Purification of Membrane Proteins in Saccharomyces cerevisiae Martin S. King and Edmund R.S. Kunji 5. Membrane Protein Expression in Insect Cells Using the Baculovirus Expression Vector System Jacques Boivineau, Matthias Haffke, and Veli-Pekka Jaakola 6. Membrane Extracts from Plant Tissues Uwe Bodensohn, Christoph Ruland, Roman Ladig, and Enrico Schleiff 7. Membrane Protein Solubilization and Quality Control: An Example of a Primary Active Transporter Charlott Stock and Inga Hänelt 8. GPCR Solubilization and Quality Control Tamara Miljus, David A Sykes, Clare Harwood, Ziva Vuckovic, and Dmitry B. Veprintsev 9. Affinity Purification of Membrane Proteins Elisabeth Graeber and Volodymyr M. Korkhov 10. Purification of Membrane Proteins by Affinity Chromatography with On-Column Protease Cleavage Stephan Hirschi and Dimitrios Fotiadis 11. Biotinylation of Membrane Proteins for Binder Selections Benedikt T. Kuhn, Iwan Zimmermann, Pascal Egloff, Lea M. Hürlimann, Cedric A.J. Hutter, Christian Miscenic, Roger J.P. Dawson, Markus A. Seeger, and Eric R. Geertsma 12. Production and Application of Nanobodies for Membrane Protein Structural Biology Janine Denise Brunner and Stephan Schenck 13. Identifying Conformation-Selective Heavy-Chain-Only Antibodies against Membrane Proteins by a Thermal-Shift Scintillation Proximity Assay Peter Stohler and Roger J.P. Dawson 14. Reconstitution of Membrane Proteins into Platforms Suitable for Biophysical and Structural Analyses Philipp A.M. Schmidpeter, Nattakan Sukomon, and Crina M. Nimigean 15. Reconstitution of Proteoliposomes for Phospholipid Scrambling and Non-Selective Channel Assays Maria E. Falzone and Alessio Accardi 16. Membrane Protein Cryo-EM: Cryo-Grid Optimization and Data Collection with Protein in Detergent Magnus Bloch, Mònica Santiveri, and Nicholas M.I. Taylor 17. Single-Particle Cryo-EM of Membrane Proteins in Lipid Nanodiscs Valeria Kalienkova, Carolina Alvadia, Vanessa Clerico Mosina, and Cristina Paulino 18. Fast Small-Scale Membrane Protein Purification and Grids Preparation for Single Particle Electron Microscopy Natalie Bärland and Camilo Perez 19. Stabilization and Crystallization of a Membrane Protein Involved in Lipids Transport Bing Zhang and Camilo Perez 20. In Meso In Situ Serial X-ray Crystallography (IMISX): A Protocol for Membrane Protein Structure Determination at the Swiss Light Source Chia-Ying Huang, Vincent Olieric, Martin Caffrey, and Meitian Wang 21. Membrane Protein Preparation for Serial Crystallography Using High Viscosity Injectors: Rhodopsin as an Example Tobias Weinert and Valérie Panneels 22. Hydrogen/Deuterium Exchange Mass Spectrometry for the Structural Analysis of Detergent-Solubilized Membrane Proteins Darragh P. O'Brien, Véronique Hourdel, Alexandre Chenal, and Sébastien Brier 23. Mechanical Unfolding and Refolding of Single Membrane Proteins by Atomic Force Microscopy Noah Ritzmann and Johannes Thoma 24. Sample Preparation and Technical Setup for NMR Spectroscopy with Integral Membrane Proteins Hundeep Kaur, Anne Grahl, Jean-Baptiste Hartmann, and Sebastian Hiller 25. Exploring Lipid- and Membrane Protein-Dynamics Using Lipid-Bilayer Nanodiscs and Solution-State NMR Spectroscopy Stefan Bibow.
• Publisher Details: New York : Humana Press
• Publication Date: 2020
• Extent: 1 online resource (xiv, 423 pages), illustrations
• Subjects: 572/.696; Membrane proteins -- Laboratory manuals; Membrane proteins; Electronic books; Laboratory manuals
• Languages: English
• ISBNs: 9781071603734; 1071603736; 1071603728; 9781071603727
• Related ISBNs: 1071603728; 9781071603727
• Notes: Note: Includes bibliographical references and index.
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.