Principles and practices of DNA analysis : a laboratory manual for forensic DNA typing /: a laboratory manual for forensic DNA typing. (2020)

• Record Type: Book
• Further Information: Note: Hirak Ranjan Dash, Pankaj Shrivastava, Surajit Das.
• Authors: Dash, Hirak Ranjan; Shrivastava, Pankaj; Das, Surajit
• Contents: Module 1: Overview of Forensic DNA analysis.- Chapter1: Introduction to Forensic DNA analysis.- Chapter 2: Biological samples: the source targets for DNA typing.- Chapter 3: Collection, transportation and preservation and of biological evidences for DNA analysis.- Module2: Manual Extraction of DNA from biological samples.- Chapter 4: Manual Isolation of DNA from whole blood.- Chapter 5. Manual Isolation of DNA from body fluid stains.- Chapter 6: Manual isolation of DNA from soft tissue sample.- Chapter7: Manual isolation of DNA from rooted hair samples.- Chapter 8: Manual Isolation of DNA from human teeth.- Chapter 9: Isolation of DNA from bone samples.- Chapter 10: Differential extraction of sperm and epithelial cell DNA.- Module 3: Automatic and semi-automatic extraction of DNA from biological samples.- Chapter 11: Isolation of DNA by using magnetic bead based extraction system.- Chapter 12: Extraction of DNA by using anion exchange resin Chelex®.- Chapter 13: Isolation of DNA by using column based extraction system.- Chapter 14. Reliable use of Whatman™ FTA™ cards for one step collection and isolation of DNA.- Module 4: Qualitative and quantitative assessment of extracted DNA.- Chapter 15: Quantification of DNA by using agarose gel electrophoresis technique.- Chapter 16: Quantification of DNA by using UV-Visible spectrophotometer.- Chapter 17: Quantification of DNA by using qRT-PCR.- Chapter 18: Fluorescence based instant quantification of DNA.- Module 5: Amplification of various STR markers using multiplex PCR.- Chapter 19: Amplification of autosomal chromosome STR markers by multiplex PCR.- Chapter 20: Amplification of Y chromosome STR markers by multiplex PCR.- Chapter 21: Amplification of X chromosome STR markers by multiplex PCR.- Chapter 22: Amplification of Autosomal Mini STR markers from degraded samples.- Module 6. Genotyping and analysis of Results.- Chapter 23: Separation of amplified DNA fragments by capillary electrophoresis using Genetic Analyzer 3500xL.- Chapter 24: Analysis of capillary electrophoresis results by GeneMapper® ID-X v 1.5 software.- Chapter 25: Calculation of paternity index in paternity dispute and identification cases.- Module 7: Case studies.- Chapter 26: DNA analysis of a disputed paternity caseSolving paternity dispute by DNA fingerprinting: A case study.- Chapter 27: Solving a case of murder by DNA fingerprinting: A case study.- Chapter 28: Solving a case of sexual assault by DNA fingerprinting: A case study.- Chapter 29: Identification of an unknown skeleton by DNA fingerprinting: A case study.- Module 8. Advanced technologies for forensic DNA analysis.- Chapter 30: Sequencing control region of human mitochondrial genome to assess matrilineal lineage.- Chapter 31: Application of Next Generation Sequencing (NGS) in forensic DNA analysis and DNA Phenotyping.- Chapter 32: Forensic Trace and Touch DNA analysis.- Chapter 33: RAPID DNA technology: A boon to forensic DNA typing.
• Publisher Details: New York, NY : Humana Press
• Publication Date: 2020
• Extent: 1 online resource (xvii, 344 pages), illustrations (some color)
• Subjects: 572.8/633; DNA fingerprinting -- Laboratory manuals; Electronic books
• Languages: English
• ISBNs: 9781071602744; 1071602748
• Related ISBNs: 9781071602737
• Notes: Note: Online resource; title from PDF title page (SpringerLink, viewed February 27, 2020).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.