RNA interference and CRISPR technologies : technical advances and new therapeutic opportunities /: technical advances and new therapeutic opportunities. ([2020])

• Record Type: Book
• Further Information: Note: Edited by Mouldy Sioud.
• Editors: Sioud, Mouldy
• Contents: RNA and CRISPR Interferences: Past, Present and Future Perspectives -- Chemical Modifications in RNA Interference and CRISPR/Cas Genome Editing Reagents -- Preparation, Determination of Activity, and Biodistribution of Cholesterol-Containing Nuclease-Resistant siRNAs In Vivo -- Multifunctional Nanodelivery Platform for Maximizing Nucleic Acids Combination Therapy -- PAMAM Dendrimers as a Delivery System for Small Interfering RNA -- Delivery of Functional Small RNAs via Extracellular Vesicles In Vitro and In Vivo -- Optimized siRNA Delivery into Primary Immune Cells using Electroporation -- Synthesis and Evaluation of Caged siRNAs with Single cRGD Modification for Photoregulating RNA Interference -- Exploring 5'-Biotinylation of the Sense Strand to Improve siRNA Specificity and Potency -- In Vivo Delivery of Cassettes Encoding Anti-HBV Primary MicroRNAs using an Ancestral Adeno-Associated Viral Vector -- Generating DNA Expression Cassettes Encoding Multimeric Artificial MicroRNA Precursors -- MiR-302-Mediated Somatic Cell Reprogramming and Method for Generating Tumor-Free iPS Cells Using miR-302 -- Urinary MicroRNAs as Emerging Class of Noninvasive Biomarkers -- Improving Dendritic Cell Cancer Vaccine Potency using RNA Interference -- Unleashing the Therapeutic Potential of Dendritic and T Cell Therapies using RNA Interference -- Harnessing the Antiviral-Type Responses Induced by Immunostimulatory siRNAs for Cancer Immunotherapy -- Cancer Immunotherapy: Targeting Tumor-Associated Macrophages by Gene Silencing -- Use of RNA Interference with TCR Transfer Enhance Safety and Efficiency -- CRISPR/Cas9 Guide RNA Design Rules for Predicting Activity -- CRISPR/Cas9 Genome Editing in Human Cell Lines with DONOR Vector Made by Gibson Assembly -- Genome Editing in Zebrafish using High-Fidelity Cas9 Nucleases: Choosing the Right Nuclease for the Task -- Next Generation of Adoptive T Cell Therapy using CRISPR/Cas9 Technology: Universal or Boosted? -- Engineering T-Cells using CRISPR/Cas9 for Cancer Therapy -- CRISPR/Cas9 Mediated Genome Engineering of Primary Human B Cells -- Gene Editing in B-Lymphoma Cell Lines using CRISPR/Cas9 Technology -- Gene Knockout in Hematopoietic Stem and Progenitor Cells followed by Granulocytic Differentiation -- CRISPR/Cas9 Genome Editing of Human Induced Pluripotent Stem Cells followed by Granulocytic Differentiation.
• Publisher Details: New York, NY : Humana Press
• Publication Date: 2020
• Extent: 1 online resource (1 PDF file (xvi, 486 pages)), illustrations (some color)
• Subjects: 611.01816; Gene expression; Genetics; RNA Interference; Clustered Regularly Interspaced Short Palindromic Repeats -- genetics; RNA, Small Interfering -- genetics; RNAi Therapeutics -- methods; Gene expression; Genetics; Laboratory Manual
• Languages: English
• ISBNs: 9781071602904; 1071602896; 9781071602898
• Related ISBNs: 107160290X; 9781071602898; 1071602896
• Notes: Note: Includes bibliographical references and index.; Note: Description based on online resource; title from PDF title page (viewed February 8, 2021).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.