DNA electrophoresis : methods and protocols /: methods and protocols. (2020)

• Record Type: Book
• Further Information: Note: Edited by Katsuhiro Hanada.
• Editors: Hanada, Katsuhiro
• Contents: Preface… Table of Contents… Contributing Authors… 1. Introduction and Perspectives of DNA Electrophoresis Katsuhiro Hanada 2. Two-Dimensional Gel Electrophoresis to Resolve DNA Topoisomers Elizabeth G. Gibson, Alexandria A. Oviatt, and Neil Osheroff 3. Using Two-Dimensional Intact Mitochondrial DNA (mtDNA) Agarose Gel Electrophoresis (2D-IMAGE) to Detect Changes in Topology Associated with Mitochondrial Replication, Transcription, and Damage Jill E. Kolesar and Brett A. Kaufman 4. 2D Gel Electrophoresis to Detect DNA Replication and Recombination Intermediates in Budding Yeast Luca Zardoni, Eleonora Nardini, and Giordano Liberi 5. Neutral-Neutral 2-Dimensional Agarose Gel Electrophoresis for Visualization of E. coli DNA Replication Structures Karla A. Mettrick, Georgia M. Weaver, and Ian Grainge 6. Alkali Comet Assay in Genotoxicity Tests Maya Ueda 7. The Analysis of DNA Interstrand Crosslinks and their Repair by the Modified Comet Assay Lonnie P. Swift, Lianne Castle, and Peter J. McHugh 8. Analysis of Chromosomal DNA Fragmentation in Apoptosis by Pulsed-Field Gel Electrophoresis Takeshi Terabayashi, Asako Tokumaru, Toshimasa Ishizaki, and Katsuhiro Hanada 9. Detection of DNA Damage-Induced DSBs by the Contour-Clamped Homogeneous Electric Field (CHEF) System in Mammalian Cells Yuri Takiguchi, Ryo Kariyazono, and Kunihiro Ohta 10. Investigation of DNA Double-Strand Breaks Induced in Host Cells following Infection with Genotoxic Bacteria Rie Teshima 11. Monitoring of DNA Replication and DNA Double-Strand Breaks in Saccharomyces cerevisiae by Pulsed-Field Gel Electrophoresis (PFGE) Kenji Keyamura and Takashi Hishida 12. Pulsed-Field Gel Electrophoresis for Detecting Chromosomal DNA Breakage in Fission Yeast Takatomi Yamada, Hiroshi Murakami, and Kunihiro Ohta 13. Detection of DNA Double-Strand Breaks by Pulsed-Field Gel Electrophoresis of Circular Bacterial Chromosomes Ichizo Kobayashi and Hanada Katsuhiro 14. Detection of Bleomycin-Induced DNA Double-Strand Breaks in Escherichia coli by Pulsed-Field Gel Electrophoresis using a Rotating Gel Electrophoresis System Naomi Inoue, Hisashi Narahara, Yoshihiro Nishida, and Katsuhiro Hanada 15. Circle-Seq: Isolation and Sequencing of Chromosome-Derived Circular DNA Elements in Cells Henrik D. Møller 16. Chromatin Pull-Down Methodology Based on DNA Triple Helix Formation Asako Isogawa, Robert P. Fuchs, and Shingo Fujii 17. DNA Fragments Agarose Gel Electrophoresis for Chromatin Immunoprecipitation (ChIP) Mayu Isono and Satoru Hashimoto 18. Post-Labeling/PAGE Method for Detection of DNA Adducts Kazuhiro Shiizaki.
• Publisher Details: New York, N.Y. : Humana Press
• Publication Date: 2020
• Extent: 1 online resource (x, 228 pages), illustrations (some color)
• Subjects: 541/.372; Electrophoresis -- Laboratory manuals; Electrophoresis -- methods; DNA; Electronic books
• Languages: English
• ISBNs: 9781071603239
• Related ISBNs: 107160323X; 1071603221; 9781071603222
• Notes: Note: Includes bibliographical references and index.
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.