RNA chaperones : methods and protocols /: methods and protocols. ([2020])

• Record Type: Book
• Further Information: Note: Edited by Tilman Heise.
• Editors: Heise, Tilman
• Contents: Biochemical Methods for the Study of the FinO Family of Bacterial RNA Chaperones -- Quantitative Analysis of RNA Chaperone Activity by Native Gel Electrophoresis and Fluorescence Spectroscopy -- Fluorescent Molecular Beacons Mimicking RNA Secondary Structures to Study RNA Chaperone Activity -- Specific Nucleic Acid Chaperone Activity of HIV-1 Nucleocapsid Protein Deduced from Hairpin Unfolding -- Real-Time Fluorescence-Based Approaches to Disentangle Mechanisms of a Protein's RNA Chaperone Activity -- Use of tRNA-Mediated Suppression to Assess RNA Chaperone Function -- Salt-Dependent Modulation of the RNA Chaperone Activity of RNA-Binding Protein La -- Kinetic and Thermodynamic Analyses of RNA-Protein Interactions -- Detection of MicroRNAs Released from Argonautes -- Mapping the RNA Chaperone Activity of the T. brucei Editosome Using SHAPE-Chemical Probing -- RNA Remodeling by RNA Chaperones Monitored by RNA Structure Probing -- In Vivo RNA Chemical Footprinting Analysis in Archaea -- RNA Structure Analysis by Chemical Probing with DMS and CMCT -- Disordered RNA Binding Region Prediction with DisoRDPbind -- Delivering Molecular Beacons via an Electroporation-Based Approach Enables Live-Cell Imaging of Single RNA Transcripts and Genomic Loci -- Site-Specific Dual-Color Labeling of Long RNAs -- Single-Molecule FRET Assay for Studying Cotranscriptional RNA Folding -- Characterizing Complex Nucleic Acid Interactions of LINE1 ORF1p by Single Molecule Force Spectroscopy -- Isolation and Analysis of Bacterial Ribosomes through Sucrose Gradient Ultracentrifugation.
• Publisher Details: New York, NY : Humana Press
• Publication Date: 2020
• Extent: 1 online resource (xi, 314 pages), illustrations
• Subjects: 611.01816; 599.935; RNA; Molecular chaperones; Human genetics; Proteins; RNA; Molecular Chaperones; RNA Folding; Chemistry Techniques, Analytical; In Vitro Techniques; Human genetics; Molecular chaperones; Proteins; RNA; Laboratory Manual
• Languages: English
• ISBNs: 9781071602317; 1071602314
• Related ISBNs: 9781071602300
• Notes: Note: Includes bibliographical references and index.; Note: Online resource; title from PDF title page (SpringerLink, viewed February 23, 2021).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.