Cytoskeleton dynamics : methods and protocols /: methods and protocols. (2020)
- Record Type:
- Book
- Title:
- Cytoskeleton dynamics : methods and protocols /: methods and protocols. (2020)
- Main Title:
- Cytoskeleton dynamics : methods and protocols
- Further Information:
- Note: Edited by Helder Maiato.
- Editors:
- Maiato, Helder
- Contents:
- 1. Purification of Mammalian Tubulins and Tubulin-Associated Proteins using a P2A-based Expression System Nuo Yu and Niels Galjart 2. Expression and Purification of Microtubule Associated Proteins from HEK293T Cells for In Vitro Reconstitution Shasha Hua and Kai Jiang 3. In vitro Reconstitution Assays of Microtubule Amplification and Lattice Repair by the Microtubule Severing Enzymes Katanin and Spastin Annapurna Vemu, Ewa Szczesna, and Antonina Roll-Mecak 4. In vitro Microtubule Dynamics Assays using Darkfield Microscopy Jeffrey O. Spector, Annapurna Vemu, and Antonina Roll-Mecak 5. In vitro Reconstitution of Dynamic Co-organization of Microtubules and Actin Laments in Emulsion Droplets Kim J.A. Vendel, Celine Alkemade, Nemo Andrea, Gijsje H. Koenderink, and Marileen Dogterom 6. Studying Tau-microtubule Interaction using Single Molecule TIRF Microscopy Virginie Stoppin-Mellet, Nassiba Bagdadi, Yasmina Saoudi, and Isabelle Arnal 7. In Vitro FRET- and Fluorescence-Based Assays to Study Protein Conformation and Protein-Protein Interactions in Mitosis Stephanie C. Ems-McClung and Claire E. Walczak 8. Methods for Studying Membrane-associated Bacterial Cytoskeleton Proteins in vivo by TIRF Microscopy Charlène Cornilleau, Arnaud Chastanet, Cyrille Billaudeau, and Rut Carballido-López 9. Processing TIRF Microscopy Images to Characterize the Dynamics and Morphology of Bacterial Actin-like Assemblies Cyrille Billaudeau, Arnaud Chastanet, and Rut Carballido-López 10. Studying1. Purification of Mammalian Tubulins and Tubulin-Associated Proteins using a P2A-based Expression System Nuo Yu and Niels Galjart 2. Expression and Purification of Microtubule Associated Proteins from HEK293T Cells for In Vitro Reconstitution Shasha Hua and Kai Jiang 3. In vitro Reconstitution Assays of Microtubule Amplification and Lattice Repair by the Microtubule Severing Enzymes Katanin and Spastin Annapurna Vemu, Ewa Szczesna, and Antonina Roll-Mecak 4. In vitro Microtubule Dynamics Assays using Darkfield Microscopy Jeffrey O. Spector, Annapurna Vemu, and Antonina Roll-Mecak 5. In vitro Reconstitution of Dynamic Co-organization of Microtubules and Actin Laments in Emulsion Droplets Kim J.A. Vendel, Celine Alkemade, Nemo Andrea, Gijsje H. Koenderink, and Marileen Dogterom 6. Studying Tau-microtubule Interaction using Single Molecule TIRF Microscopy Virginie Stoppin-Mellet, Nassiba Bagdadi, Yasmina Saoudi, and Isabelle Arnal 7. In Vitro FRET- and Fluorescence-Based Assays to Study Protein Conformation and Protein-Protein Interactions in Mitosis Stephanie C. Ems-McClung and Claire E. Walczak 8. Methods for Studying Membrane-associated Bacterial Cytoskeleton Proteins in vivo by TIRF Microscopy Charlène Cornilleau, Arnaud Chastanet, Cyrille Billaudeau, and Rut Carballido-López 9. Processing TIRF Microscopy Images to Characterize the Dynamics and Morphology of Bacterial Actin-like Assemblies Cyrille Billaudeau, Arnaud Chastanet, and Rut Carballido-López 10. Studying Centriole Duplication and Elongation in Human Cells Catarina Peneda, Carla A.M. Lopes, and Mónica Bettencourt-Dias 11. Assaying Microtubule Nucleation Artur Ezquerra, Ricardo Viais, and Jens Lüders 12. Detection of Microtubule Nucleation Hotspots at the Golgi Roslin J. Thoppil, Anna A.W.M Sanders, and Irina Kaverina 13. Ultrastructural Analysis of Microtubule Ends J. Richard McIntosh, Eileen O'Toole, Cynthia Page, and Garry Morgan 14. Optogenetic Control of Microtubule Dynamics Jeffrey van Haren, Lauren Adachi, and Torsten Wittmann 15. Measurement of Microtubule Half-Life and Poleward Flux in the Mitotic Spindle by Photoactivation of Fluorescent Tubulin Hugo Girão and Helder Maiato 16. Functional Dissection of Mitosis using Immortalized Fibroblasts from the Indian Muntjac, A Placental Mammal with only Three Chromosomes Ana C. Almeida, Danica Drpic, Naoyuki Okada, Joana Bravo, Marta Madureira, and Helder Maiato 17. Visualization and Functional Analysis of Spindle Actin and Chromosome Segregation in Mammalian Oocytes Binyam Mogessie 18. Using the Four-cell C. elegans Embryo to Study Contractile Ring Dynamics during Cytokinesis Fung Yi Chan, Ana Marta Silva, and Ana Xavier Carvalho 19. Knocking out Multiple Genes in Cultured Primary Neurons to Study Tubulin Posttranslational Modifications Satish Bodakuntla, Carsten Janke, and Maria M. Magiera 20. Measuring the Role of Tubulin Posttranslational Modifications on Axonal Transport Satish Bodakuntla, Maria M. Magiera, and Carsten Janke 21. Methods to Measure Perinuclear Actin Dynamics during Nuclear Movement in Migrating Cells Cátia S. Janota, Francisco J. Calero-Cuenca, and Edgar R. Gomes. … (more)
- Publisher Details:
- New York, NY : Humana Press
- Publication Date:
- 2020
- Extent:
- 1 online resource (xi, 390 pages), illustrations (some color)
- Subjects:
- 571.6/54
Cytoskeleton -- Laboratory manuals
Electronic books - Languages:
- English
- ISBNs:
- 9781071602195
1071602195 - Related ISBNs:
- 9781071602188
- Notes:
- Note: Online resource; title from PDF title page (SpringerLink, viewed January 15, 2020).
- Access Rights:
- Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
- Access Usage:
- Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.
- View Content:
- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library HMNTS - ELD.DS.479998
- Ingest File:
- 03_031.xml