MERS coronavirus : methods and protocols /: methods and protocols. (2020)

• Record Type: Book
• Further Information: Note: Edited by Rahul Vijay.
• Editors: Vijay, Rahul
• Contents: PART I: Evolution and Entry of MERS-Coronavirus 1. Studying Evolutionary Adaptation of MERS-CoV Michael Letko and Vincent Munster 2. Evaluating MERS-CoV Entry Pathways Enya Qing, Michael P Hantak, Gautami G Galpalli, and Tom Gallagher 3. Biochemical Characterization of Middle East Respiratory Syndrome Coronavirus Spike Protein Proteolytic Processing Gary R. Whittaker and Jean K. Millet 4. Crystallization and Structural Determination of the Receptor-binding Domain of MERS-CoV Spike Glycoprotein Haixia Zhou, Shuyuan Zhang, and Xinquan Wang PART II: Genetic Alteration and Structural Determination of MERS-Coronavirus Proteins 5. Bacterial Artificial Chromosome-based Lambda Red Recombination with the I-SceI Homing Endonuclease for Genetic Alteration of MERS-CoV Anthony R. Fehr 6. Deducing the Crystal Structure of MERS-CoV Helicase Sheng Cui and Wei Hao PART III: Quantitation of Virus and Anti-Viral Factors 7. Antigen Capture Enzyme-linked Immunosorbent Assay for Detecting Middle East Respiratory Syndrome Coronavirus in Humans Joshua Fung, Susanna K. P. Lau, and Patrick C. Y. Woo 8. Quantification of the Middle East Respiratory Syndrome-Coronavirus RNA in Tissues by Quantitative Real-Time RT-PCR Abdullah Algaissi, Anurodh S. Agrawal, Anwar M. Hashem, and Chien-Te K. Tseng 9. Evaluation of MERS-CoV Neutralizing Antibodies in Sera Using Live Virus Microneutralization Assay Abdullah Algaissi and Anwar M Hashem 10. Generation of MERS-CoV Pseudotyped Viral Particles for the Evaluation of Neutralizing Antibodies in Mammalian Sera Abdulrahman Almasaud, Naif Khalaf Alharbi, and Anwar M Hashem 11. Qualitative and Quantitative Determination of MERS-CoV S1-specific Antibodies using ELISA Sawsan S Al-amri and Anwar M Hashem PART IV: Mouse Models for MERS –Coronavirus 12. Genetically Engineering a Susceptible Mouse Model for MERS-CoV-Induced Acute Respiratory Distress Syndrome Sarah R. Leist and Adam S. Cockrell 13. Development of a Mouse Adapted MERS Coronavirus Kun Li and Paul B. McCray, Jr. 14. Metabolite, Protein, and Lipid Extraction (MPLEx): A Method that Simultaneously Inactivates Middle East Respiratory Syndrome Coronavirus and Allows Analysis of Multiple Host Cell Components Following Infection Carrie D. Nicora, Amy C. Sims, Kent J. Bloodsworth, Young-Mo Kim, Ronald J. Moore, Jennifer E. Kyle, Ernesto S. Nakayasu, and Thomas O. Metz 15. Evaluation of Activation and Inflammatory Activity of Myeloid Cells During Pathogenic Human Coronavirus Infection Rudragouda Channappanavar and Stanley Perlman 16. Histopathologic Evaluation and Scoring of Viral Lung Infection David K. Meyerholz and Amanda P. Beck.
• Publisher Details: New York, NY : Humana Press
• Publication Date: 2020
• Extent: 1 online resource (xi, 224 pages), illustrations (some color)
• Subjects: 579.2/56; MERS (Disease) -- Laboratory manuals; Electronic books
• Languages: English
• ISBNs: 9781071602119
• Related ISBNs: 107160211X; 9781071602102
• Notes: Note: Online resource; title from PDF title page (SpringerLink, viewed January 15, 2020).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.