Fundamental approaches to screen abnormalities in drosophila. (c2020)

• Record Type: Book
• Further Information: Note: Edited by Monalisa Mishra.
• Other Names: Mishra, Monalisa
• Contents: Chapter 1. Preparation of normal food and modified food for Drosophila -- Chapter 2. Protocol for dissection of various tissues (gut, imaginal disc, fat body, ovary, testes) in larva and adult fly -- Chapter 3. Biochemical estimation of various metabolites (protein, lipid, carbohydrate, trehalose, cholesterol) -- Chapter 4. Detection of fat within the Drosophila body -- Chapter 5. Estimation of reactive oxygen species (ROS) production in Drosophila cells -- Chapter 6. Methods to check the metabolic activity of fly -- Chapter 7. Detection of DNA damage in larva and adult stage of Drosophila by Fluorescent microscopy -- Chapter 8. Imaging of larva/adult gut damage using Bright field microscopy -- Chapter 9. A comet assay to detect severity of DNA damage -- Chapter 10. Checking the abnormality in hemolymph by Giemsa stain -- Chapter 11. Analysis of channel proteins -- Chapter 12. Various behavioral assays in larva and adult Drosophila -- Chapter 13. Detection of various metals within the tissue by Energy dispersive X-ray (EDX) through SEM and FTIR -- Chapter 14. Imaging of various sections of fly through Scanning Electron Microscopy (SEM) -- Chapter 15. Preparation of thin sections and fixation of various tissues of Drosophila for examination by transmission electron microscopy (TEM) -- Chapter 16. Flow cytometric analysis of Drosophila cells.
• Publisher Details: New York : Springer
• Publication Date: 2020
• Copyright Date: 2020
• Extent: 1 online resource
• Subjects: 595.77/4; Drosophila -- Laboratory manuals; Drosophila -- Genetics -- Laboratory manuals; Drosophila; Drosophila -- Genetics; Electronic books; Laboratory manuals
• Languages: English
• ISBNs: 9781493997565; 1493997564; 1493997556; 9781493997558
• Related ISBNs: 1493997556; 9781493997558
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.