Label-free monitoring of cells in vitro. (©2019)

• Record Type: Book
• Further Information: Note: Joachim Wegener, editor ; with contributions by F. Alexander Jr. [and 22 others].
• Authors: Jr, Alexander, F (Frank)
• Editors: Prof, Wegener, Joachim
• Contents: Intro; Preface; Contents; Impedance-Based Assays Along the Life Span of Adherent Mammalian Cells In Vitro: From Initial Adhesion to Cell Death; 1 Introduction; 2 Physical Background of Impedance-Based Assays; 2.1 Behavior of Cells in Electric Fields; 2.2 Physical Basics of Impedance Sensing; 2.3 Experimental Setup for Impedance Measurements of Adherent Cells In Vitro; 2.4 Popular Electrode Designs and Commercial Systems; 2.5 The Measurement Principle; 2.6 The ECIS Model: Correlating Impedance Readings with Cell Morphology 3 Impedance-Based Assays to Study Cell Phenotypes and Behavior In Vitro3.1 Cell-Matrix Adhesion; 3.2 Cell Proliferation; 3.3 Cell Layer Maturation: Barrier Function as a Prominent Example; 3.4 Micromotion: An Indicator for Viability and Motility; 3.5 Cell Migration: The Electrical Wound-Healing Assay and 3D Approaches; 3.6 Morphology Changes in Response to Biological, Chemical, or Physical Stimuli; 3.6.1 Receptor Activation and Signal Transduction; Impedance-Based Assays in Receptor-Mediated Signaling Research; Impedance-Based Assays in Drug Discovery Targeting Cell Surface Receptors 3.6.2 Cell Differentiation3.6.3 Cell-Cell and Cell-Pathogen Interactions; 3.6.4 Response to Physical Challenges: Invasive Electric Fields, Fluid Shear, and More; 3.7 Monitoring Cell Death and Cytotoxicity; 4 Concluding Remarks; References; Transistor-Based Impedimetric Monitoring of Single Cells; 1 Introduction; 2 Materials and Methods; 2.1 Field-Effect Transistors; 2.2 Measurement Setups; 2.3 Chip Encapsulation; 2.4 Preparation of Devices and Cells; 3 Results and Discussion; 3.1 Low Density Cultures; 3.2 Single Cell Impedance; 3.3 TTF Spectra and Modeling 3.4 Optimization of Devices for Single Cell FETCIS3.5 Recordings from Primary Neuronal Cultures; 3.6 Human T-Cell Experiments; 3.7 Adjustment of Medium Conductivity for Improved FETCIS; 4 Conclusion and Outlook; References; Label-Free Monitoring of 3D Tissue Models via Electrical Impedance Spectroscopy; 1 Introduction; 1.1 Microelectrode Design and Spatial Resolution; 1.2 Optimization of Measurement Parameters; 1.3 Literature Review on the Optimization of Electrode Design; 2 Real-Time Monitoring of 3D Tissue Models; 2.1 Scaffold-Based 3D Cultures; 2.2 EIS in Scaffold-Based 3D Cultures 2.3 Scaffold-Free 3D Cultures2.4 EIS in Scaffold-Free 3D Cultures; 3 A Linear Electrode Array Integrated in a Microfluidic Channel for Spheroid Measurements; 4 Modeling of 3D Microelectrodes; 5 The Future of EIS-Based Monitoring of 3D Tissue Models; References; On the Use of the Quartz Crystal Microbalance for Whole-Cell-Based Biosensing; 1 Introduction; 2 Instrumental Aspects; 3 Background: The Non-gravimetric QCM; 3.1 Full-Fledged Viscoelastic Modeling Is Difficult for Biological Cells; 4 Simple Models and Situations: Where These Can Be Applied
• Publisher Details: Cham : Springer
• Publication Date: 2019
• Copyright Date: 2019
• Extent: 1 online resource (286 pages)
• Subjects: 571.638; Human cell culture; Biological assay; Biological assay; Human cell culture; Electronic books; Electronic books
• Languages: English
• ISBNs: 9783030324339; 3030324338
• Related ISBNs: 303032432X; 9783030324322
• Notes: Note: Includes bibliographical references and index.; Note: Online resource; title from PDF title page (SpringerLink, viewed December 4, 2019).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.