Chimeric RNA : methods and protocols /: methods and protocols. (2020)

• Record Type: Book
• Further Information: Note: Edited by Hui Li, Justin Elfman.
• Editors: Li, Hui; Elfman, Justin
• Contents: Prediction, Characterization, and In Silico Validation of Chimeric RNAs -- Identification of Fusion Transcripts from Unaligned RNA-Seq Reads Using ChimeRScope -- Identification of Chimeric RNAs Using RNA-Seq Reads and Protein-Protein Interactions of Translated Chimeras -- Gene Fusion Discovery with INTEGRATE -- Case Study: Systematic Detection and Prioritization of Gene Fusions in Cancer by RNA-Seq: A DIY Toolkit -- Detection and Measurement of Chimeric RNAs by RT-PCR -- Detection of Group II Intron-Generated Chimeric mRNAs in Bacterial Cells -- RNase Protection Assay -- Validation of Chimeric Fusion Peptides Using Proteomics Data -- NanoString nCounter Technology: High-Throughput RNA Validation -- Knockdown of Chimeric RNA by RNAi -- Overexpression of Chimeric RNA by Retroviral Transduction -- Separation of Nuclear and Cytoplasmic Fractions for Chimeric RNA Characterization -- Confirmation of Transcriptional Read-Through Events by RT-PCR -- Validating Gene Fusion as the Source of Chimeric RNAs -- Chimeric RNA and Exosomes-Based Liquid Biopsy -- SMaRT for Therapeutic Purposes -- Case Study: Landscape of Chimeric RNAs in Bladder Cancer -- Case Study: The Recurrent Fusion RNA DUS4L-BCAP29 in Non-Cancer Human Tissues and Cells.
• Publisher Details: New York, NY : Humana
• Publication Date: 2020
• Extent: 1 online resource (xii, 262 pages. 56 illustrations, 52 illustrations in color.)
• Subjects: 616.042; Chimerism; RNA; Genetics and Genomics; Gene Function; Cancer Research; Electronic books
• Languages: English
• ISBNs: 9781493999040; 1493999044
• Related ISBNs: 9781493999033
• Notes: Note: Online resource; title from PDF title page (SpringerLink, viewed November 18, 2019).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.