Histidine phosphorylation : methods and protocols /: methods and protocols. ([2020])

• Record Type: Book
• Further Information: Note: Edited by Claire E. Eyers.
• Editors: Eyers, Claire E
• Contents: 1. Protein Dynamics in Phosphoryl-transfer Signaling Mediated by Two-component Systems Felipe Trajtenberg and Alejandro Buschiazzo 2. A High-throughput Strategy for Recombinant Protein Expression and Solubility Screening in Escherichia coli – A Case of Sensor Histidine Kinase Agnieszka Szmitkowska, Blanka Pekárová, and Jan Hejátko 3. SDS-PAGE and Dot Blot Autoradiography: Tools for Quantifying Histidine Kinase Autophosphorylation Jonathan T. Fischer, Ilana Heckler, and Elizabeth M. Boon 4. A Quantitative Method for the Measurement of Protein Histidine Phosphorylation Paul V. Attwood 5. Analysis of 1- and 3-phosphohistidine (pHis) Protein Modification using Model Enzymes Expressed in Bacteria Alice K. M. Clubbs Coldron, Dominic P. Byrne, and Patrick A. Eyers 6. Determination of Phosphohistidine Stoichiometry in Histidine Kinases by Intact Mass Spectrometry Lauren J. Tomlinson, Alice K. M. Clubbs Coldron, Patrick A. Eyers, and Claire E. Eyers 7. Protein Phosphohistidine Phosphatases of the HP Superfamily Daniel J. Rigden 8. In Vitro Assays for Measuring Protein Histidine Phosphatase Activity Brandon S. McCullough and Amy M. Barrios 9. Structural and Functional Characterization of Autophosphorylation in Bacterial Histidine Kinases Laura Miguel-Romero, Cristina Mideros-Mora, Alberto Marina, and Patricia Casino 10. Manipulation of Bacterial Signalling using Engineered Histidine Kinases Kimberly A. Kowallis, Samuel W. Duvall, Wei Zhao, and W. Seth Childers 11. Development of a Light-Dependent Protein Histidine Kinase Aleksandra Bury and Klaas J. Hellingwerf 12. Empirical Evidence of Cellular Histidine Phosphorylation by Immunoblotting using pHis mAbs Rajasree Kalagiri, Kevin Adam, and Tony Hunter 13. Immunohistochemistry (IHC): Chromogenic Detection of 3-Phosphohistidine Proteins in Formaldehyde-Fixed, Frozen Mouse Liver Tissue Sections Natalie Luhtala and Tony Hunter 14. Subcellular Localisation of Histidine Phosphorylated Proteins through Indirect Immunofluorescence Kevin Adam and Tony Hunter 15. High throughput Characterisation of Histidine Phosphorylation sites using UPAX and Tandem Mass Spectrometry Gemma Hardman and Claire E. Eyers 16. Proteome Bioinformatics Methods for Studying Histidine Phosphorylation Andrew R Jones and Oscar Martin Camacho.
• Publisher Details: New York : Humana Press
• Publication Date: 2020
• Copyright Date: 2020
• Extent: 1 online resource, illustrations (some color)
• Subjects: 547/.2; Phosphorylation; Amino acids; Electronic books
• Languages: English
• ISBNs: 9781493998845; 1493998846
• Related ISBNs: 9781493998838
• Notes: Note: Online resource; title from PDF title page (SpringerLink, viewed November 14, 2019).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.