Antibody-drug conjugates : methods and protocols /: methods and protocols. ([2020])

• Record Type: Book
• Further Information: Note: Edited by L. Nathan Tumey.
• Editors: Tumey, L. Nathan
• Contents: Preface… Table of Contents… Contributing Authors… 1. An Overview of the Current ADC Discovery Landscape L. Nathan Tumey 2. Pushing the Envelope: Advancement of ADCs Outside of Oncology Michael J. McPherson and Adrian D. Hobson 3. Conjugations to Endogenous Cysteine Residues Durgesh V. Nadkarni 4. Site-Specific Conjugation to Cys-Engineered THIOMAB™ Antibodies Pragya Adhikari, Neelie Zacharias, Rachana Ohri, and Jack Sadowsky 5. Transglutaminase-Mediated Conjugations Yasuaki Anami and Kyoji Tsuchikama 6. Click Chemistry Conjugations Tak Ian Chio and Susan L. Bane 7. Utilizing Solid-Phase to Enable High-Throughput, Site-Specific Conjugation and Dual Labeled Antibody and Fab Conjugates Sujiet Puthenveetil 8. Bridged Cysteine Conjugations Mark Frigerio, Joao Nunes, and Matthew Bird 9. Antibody Conjugations via Glycosyl Remodeling Hanna Toftevall, Helén Nyhlén, Fredrik Olsson, and Jonathan Sjögren 10. ADC Analysis by Hydrophobic Interaction Chromatography Ryan Fleming 11. Two-Dimensional Liquid Chromatography Coupled to High Resolution Mass Spectrometry for the Analysis of ADCs Soraya Chapel, Florent Rouvière, Morgan Sarrut, and Sabine Heinisch 12. Drug Loading and Distribution of ADCs after Reduction or IdeS Digestion and Reduction Elsa Wagner-Rousset, Olivier Colas, Yannis François, Sabine Heinisch, Davy Guillarme, Sarah Cianférani, and Alain Beck 13. Analysis of ADCs by Native Mass Spectrometry Oscar Hernandez-Alba, Anthony Ehkirch, Alain Beck, and Sarah Cianférani 14. High Resolution Characterization of ADCs by Orbitrap LCMS Jintang He, Surinder Kaur, and Keyang Xu 15. Conjugation Site Analysis by MS/MS Protein Sequencing Linjie Han, Yanqun Zhao, and Qunying Zhang 16. Conjugation Site Analysis of Lysine-Conjugated ADCs Hua Sang, Ning Wan, Gaoyuan Lu, Yang Tian, Guangji Wang, and Hui Ye 17. Characterization of ADCs by Capillary Electrophoresis Wenjing Ning and Yanqun Zhao 18. Characterization of the Primary Structure of Cysteine-Linked Antibody-Drug Conjugates using Capillary Electrophoresis with Mass Spectrometry Josiane Saadé, Rabah Gahoual, Alain Beck, Emmanuelle Leize-Wagner, and Yannis-Nicolas François 19. Purification of ADCs by Hydrophobic Interaction Chromatography Calvin L. Becker, Robert J. Duffy, Jorge Gandarilla, and Steven M. Richter 20. Detection and Removal of Small-Molecule and Endotoxin Contaminants in ADC Preparations Jeffery Casavant, Anokha S. Ratnayake, Sujiet Puthenveetil, and L. Nathan Tumey 21. Physical Stability Studies of Antibody Drug Conjugates (ADCs) Under Stressed Conditions Yilma T. Adem 22. Biophysical Methods for Characterization of Antibody Drug Conjugates Vamsi Krishna Mudhivarthi and Jianxin Guo 23. Determination of ADC Cytotoxicity in Immortalized Human Cell Lines Shengjia Wu and Dhaval K. Shah 24. LC/MS Methods for Studying Lysosomal ADC Catabolism Andrew J. Bessire and Chakrapani Subramanyam 25. Assessing ADC Plasma Stability by LC/MS Methods Cong Wei 26. Determination of ADC Concentration by Ligand-Binding Assays Hsuan-Ping Chang and Dhaval K. Shah.
• Publisher Details: New York, NY : Humana Press
• Publication Date: 2020
• Extent: 1 online resource (xi, 373 pages), illustrations (some color)
• Subjects: 615.3/6; Antibody-drug conjugates; Electronic books
• Languages: English
• ISBNs: 9781493999293
• Related ISBNs: 149399929X; 9781493999286
• Notes: Note: Online resource; title from PDF title page (SpringerLink, viewed October 29, 2019).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.