Genotype phenotype coupling : methods and protocols /: methods and protocols. ([2020])

• Record Type: Book
• Further Information: Note: Edited by Stefan Zielonka and Simon Krah.
• Editors: Zielonka, Stefan; Krah, Simon
• Contents: 1. Screening, Post-SELEX Optimization and Application of DNA Aptamers Specific for Tobramycin Nandi Zhou, Rongfeng Cai, and Xuyan Han 2. Affitins: Ribosome Display for Selection of Aho7c-based Affinity Proteins Valentina Kalichuk, Stanimir Kambarev, Ghislaine Béhar, Benjamin Chalopin, Axelle Renodon-Cornière, Barbara Mouratou, and Frédéric Pecorari 3. cDNA Display: A Stable and Simple Genotype–phenotype Coupling Using a Cell-free Translation System Hidenao Arai, Shigefumi Kumachi, and Naoto Nemoto 4. cDNA Display of Disulfide-containing Peptide Library and in vitro Evolution Tai Kubo and Mohammed Naimuddin 5. Rapid Antigen and Antibody-like Molecule Discovery by Staphylococcal Surface Display Marco Cavallari 6. Restriction-free Construction of a Phage-presented Very Short Macrocyclic Peptide Library Valentin Jakob, Saskia Helmsing, Michael Hust, and Martin Empting 7. In Vitro Maturation of a Humanized Shark VNAR Domain to Improve Its Biophysical Properties John Steven, Obinna C. Ubah, Magdalena Buschhaus, Marina Kovaleva, Laura Ferguson, Andrew J. Porter, and Caroline J. Barelle 8. Antibody Phage Display: Antibody Selection in Solution Using Biotinylated Antigens Esther V. Wenzel, Kristian D. R. Roth, Giulio Russo, Viola Fühner, Saskia Helmsing, André Frenzel, and Michael Hust 9. Assessing Antibody Specificity in Human Serum Using Deep Sequence-coupled Biopanning Kathryn M. Frietze, Susan B. Core, Alexandria Linville, Bryce Chackerian, and David S. Peabody 10. Isolation of Antigen-specific VHH Single Domain Antibodies by Combining Animal Immunization with Yeast Surface DisplayLukas Roth, Simon Krah, Janina Klemm, Ralf Guenther, Lars Toleikis, Michael Busch, Stefan Becker, and Stefan Zielonka 11. Selection and Characterization of Anti-idiotypic Shark Antibody Domains Doreen Könning, Stefan Zielonka, Anna Kaempffe, Sebastian Jäger, Harald Kolmar, and Christian Schröter 12. Simplifying the Detection of Surface Presentation Levels in Yeast Surface Display by Intracellular tGFP Expression Steffen C. Hinz, Adrian Elter, Julius Grzeschik, Jan Habermann, Bastian Becker, and Harald Kolmar 13. Methods for Construction of Yeast Display Libraries of Four-Domain T-cell Receptors Flávio Sádio, Gerhard Stadlmayr, Katja Eibensteiner, Katharina Stadlbauer, Florian Rüker, and Gordana Wozniak-Knopp 14. Isolation of Tailor-made Antibody Fragments from Yeast-displayed B-Cell Receptor Repertoires by Multiparameter Fluorescence-Activated Cell Sorting Anna Kaempffe, Sebastian Jäger, Doreen Könning, Harald Kolmar, and Christian Schröter 15. Isolation of Anti-Hapten Antibodies by Fluorescence-Activated Cell Sorting of Yeast-Displayed B-Cell Receptor Gene Repertoires Sebastian Jäger, Simon Krah, Doreen Könning, Janis Rosskopf, Stephan Dickgiesser, Nicolas Rasche, Harald Kolmar, Stefan Hecht, and Christian Schröter 16. Rapid Generation of Chicken Immune Libraries for Yeast Surface Display Jan P. Bogen, Julius Grzeschik, Simon Krah, Stefan Zielonka, and Harald Kolmar 17. Ligand Engineering via Yeast Surface Display and Adherent Cell Panning Lawrence A. Stern, Patrick S. Lown, and Benjamin J. Hackel 18. Simultaneous Soluble Secretion and Surface Display of Proteins in Saccharomyces Cerevisiae Using Inefficient Ribosomal Skipping Carlos A. Cruz-Teran, Kaitlyn Bacon, and Balaji M. Rao 19. Chemical Modification of the Yeast Cell Surface Allows the Switch between Display and Soluble Secretion of Full-length Antibodies Simon Krah, Ralf Günther, Stefan Becker, Stefan Zielonka, and Laura Rhiel 20. Advanced Establishment of Stable Recombinant Human Suspension Cell Lines Using Geno-Phenotype Coupling Transposon Vectors Karen Berg, Vanessa Nicole Schäfer, Natalie Bartnickiand, and Jörn Stitz 21. Mammalian Surface Display Screening of Diverse Cystine-Dense Peptide Libraries for Difficult to Drug TargetsZachary R. Crook, Gregory P. Sevilla, Andrew J. Mhyre, and James M. Olson 22. Engineering Antibodies on the Surface of CHO Cells Annalee W. Nguyen, Kevin Le, and Jennifer A. Maynard23. Single B Cell Cloning and Production of Rabbit Monoclonal AntibodiesJuliet Rashidian and Joshua Lloyd.
• Publisher Details: New York : Humana Press
• Publication Date: 2020
• Copyright Date: 2010
• Extent: 1 online resource, illustrations (some color)
• Subjects: 660.6/3; Protein engineering; Phenotype; Electronic books
• Languages: English
• ISBNs: 9781493998531; 1493998536
• Related ISBNs: 9781493998524
• Notes: Note: Online resource; title from PDF title page (SpringerLink, viewed October 25, 2019).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.