Trinucleotide repeats : methods and protocols /: methods and protocols. (2020)

• Record Type: Book
• Further Information: Note: Edited by Guy-Franck Richard.
• Editors: Richard, Guy-Franck
• Contents: 1. Experimenting with Trinucleotide Repeats: Facts and Technical Issues Guy-Franck Richard 2. Fast Assays to Detect Interruptions in CTG.CAG Repeat Expansions Stéphanie Tomé and Geneviève Gourdon 3. Tracking Expansions of Stable and Threshold Length Trinucleotide Repeat Tracts In Vivo and In Vitro Using Saccharomyces cerevisiae Gregory M. Williams, Athena K. Petrides, Lata Balakrishnan, and Jennifer A. Surtees 4. Quantifying Replication Fork Progression at CTG Repeats by 2D Gel Electrophoresis David Viterbo and Guy-Franck Richard 5. Genetic Assays to Study Repeat Fragility in Saccharomyces cerevisiae Erica J. Polleys and Catherine H. Freudenreich 6. Genetic Screens to Study GAA/TTC and Inverted Repeat Instability in Saccharomyces cerevisiae Wenying Guo and Kirill S. Lobachev 7. Monitoring Double-Strand Break Repair of Trinucleotide Repeats Using a Yeast Fluorescent Reporter Assay Lucie Poggi, Bruno Dumas, and Guy-Franck Richard 8. Analysis of Trinucleotide Repeat Stability by Integration at a Chromosomal Ectopic Site Rujuta Yashodhan Gadgil, S. Dean Rider, Todd Lewis, Joanna Barthelemy, and Michael Leffak 9. Experimental System to Study Instability of (CGG)n Repeats in Cultured Mammalian Cells Artem V. Kononenko, Thomas Ebersole, and Sergei M. Mirkin 10. Assessing Triplet Repeat Expansions in Human SVG-A Cell Culture Gregory M. Williams and Robert S. Lahue 11. The Isolation and Analysis of the CGG-Repeat Size in Male and Female Gametes from a Fragile X Mouse Model Xiaonan Zhao, Huiyan Lu, Pradeep K. Dagur, and Karen Usdin 12. In Vitro Synthesis and RNA Structure Probing of CUG Triplet Repeat RNA Remco T.P. van Cruchten and Derick G. Wansink 13. FISH Protocol for Myotonic Dystrophy Type 1 Cells Arnaud F. Klein, Ludovic Arandel, Joelle Marie, and Denis Furling 14. Real Time Videomicroscopy and Semi-Automated Analysis of Brain Cell Culture Models of Trinucleotide Repeat Expansion Diseases Sandra O. Braz, Diana M. Dinca, Geneviève Gourdon, and Mário Gomes-Pereira 15. Primary Cultures of Pure Embryonic Dorsal Root Ganglia Sensory Neurons as a New Cellular Model for Friedreich's Ataxia Olivier Griso and Hélène Puccio 16. GFP Reporters to Monitor Instability and Expression of Expanded CAG/CTG Repeats Cinzia Cinesi, Bin Yang, and Vincent Dion 17. Gene Therapy for Huntington's Disease Using Targeted Endonucleases Magdalena Dabrowska and Marta Olejniczak.
• Publisher Details: New York, NY : Humana Press
• Publication Date: 2020
• Extent: 1 online resource (xi, 286 pages), illustrations (some color)
• Subjects: 572.8/5; Trinucleotide repeats -- Laboratory manuals; Electronic books
• Languages: English
• ISBNs: 9781493997848
• Related ISBNs: 149399784X; 9781493997831
• Notes: Note: Online resource; title from PDF title page (SpringerLink, viewed October 8, 2019).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.