Brain development : methods and protocols /: methods and protocols. (2020)

• Record Type: Book
• Further Information: Note: Edited by Simon G. Sprecher.
• Editors: Sprecher, Simon G
• Contents: Part I: Invertebrate Models 1. Combining BrdU-Labeling to Detection of Neuronal Markers to Monitor Adult Neurogenesis in Hydra Wanda Buzgariu, Marie-Laure Curchod, Chrystelle Perruchoud, and Brigitte Galliot 2. Reverse Genetic Approaches to Investigate the Neurobiology of the Cnidarian Sea Anemone Nematostella vectensis Jamie A. Havrilak and Michael J. Layden 3. Generating Transgenic Reporter Lines for Studying Nervous System Development in the Cnidarian Nematostella vectensis Fabian Rentzsch, Eduard Renfer, and Ulrich Technau 4. Immunostaining and In Situ Hybridization of the Developing Acoel Nervous System Elena Perea-Atienza, Brenda Gavilán, Simon G. Sprecher, and Pedro Martinez 5. Immunostaining of the Embryonic and Larval Drosophila Brain Frank Hirth and Danielle C. Diaper 6. Non-Fluorescent RNA In Situ Hybridization Combined with Antibody Staining to Visualize Multiple Gene Expression Patterns in the Embryonic Brain of Drosophila David Jussen and Rolf Urbach 7. Analysis of Complete Neuroblast Cell Lineages in the Drosophila Embryonic Brain via DiI Labeling Karoline F. Kraft and Rolf Urbach 8. Flybow to Dissect Circuit Assembly in the Drosophila Brain: An Update Emma L. Powell and Iris Salecker 9. Live Cell Imaging of Neural Stem Cells in the Drosophila Larval Brain Karolina Miszczak and Boris Egger 10. CRISPR/Cas9 Genome Editing to Study Nervous System Development in Drosophila Cornelia Fritsch and Simon G. Sprecher 11. The Red Flour Beetle as Model for Comparative Neural Development: Genome Editing to Mark Neural Cells in Tribolium Brain Development Max S. Farnworth, Kolja N. Eckermann, Hassan M.M. Ahmed, Dominik S. Mühlen, Bicheng He, and Gregor Bucher 12. A Protocol for Double Fluorescent In Situ Hybridization and Immunohistochemistry for the Study of Embryonic Brain Development in Tribolium castaneum Marita Büscher, Georg Oberhofer, Natalia Carolina Garcia-Perez, and Gregor Bucher 13. Immunohistochemistry and Fluorescent Whole Mount RNA In Situ Hybridization in Larval and Adult Brains of Tribolium Vera S. Hunnekuhl, Max S. Farnworth, Bicheng He, and Gregor Bucher 14. X-Ray Microscopy of the Larval Crustacean Brain Jakob Krieger and Franziska Spitzner 15. Immunolocalization of Neurotransmitters and Neuromodulators in the Developing Crayfish Brain Steffen Harzsch and Caroline Viertel 16. Immunostainings in Nervous System Development of the Nematode C. elegans Janet S. Duerr 17. Methods in Brain Development of Molluscs Andreas Wanninger and Tim Wollesen 18. A Simple Method to Identify Ascidian Brain Lineage Cells at Neural Plate Stages Following In Situ Hybridization Clare Hudson 19. Spawning Induction and Embryo Micromanipulation Protocols in the Amphioxus Branchiostoma lanceolatum Yann Le Petillon, Stéphanie Bertrand, and Héctor Escrivà Part II: Vertebrate Models 20. In Situ Hybridization and Immunostaining of Xenopus Brain Kai-li Liu, Xiu-mei Wang, Zi-long Li, Ying Liu, and Rong-qiao He 21. Morpholino Studies in Xenopus Brain Development Jennifer E. Bestman and Hollis T. Cline 22. Sensitive Multiplexed Fluorescent In Situ Hybridization Using Enhanced Tyramide Signal Amplification and Its Combination with Immunofluorescent Protein Visualization in Zebrafish Gilbert Lauter, Iris Söll, and Giselbert Hauptmann 23. Live Morphometric Classification of Sensory Neurons in Larval Zebrafish Gema Valera and Hernán López-Schier 24. Immunohistochemistry and In Situ Hybridization in the Developing Chicken Brain Richard P. Tucker, Tatsuto Ishimaru, and Qizhi Gong 25. Gene Silencing in Chicken Brain Development Georgia Tsapara, Irwin Andermatt, and Esther T. Stoeckli 26. Transplantation of Neural Tissue: Quail-Chick Chimeras Andrea Streit and Claudio D. Stern 27. Immunohistochemistry and RNA In Situ Hybridization in Mouse Brain Development Jinling Liu and Aimin Liu 28. The Cre/Lox System to Assess the Development of the Mouse Brain Claudius F. Kratochwil and Filippo M. Rijli 29. In Utero Electroporation to Study Mouse Brain Development Emilie Pacary and François Guillemot.
• Edition: Second edition
• Publisher Details: New York, NY : Humana Press
• Publication Date: 2020
• Extent: 1 online resource (xiv, 527 pages), illustrations (some color)
• Subjects: 573.8/6; Brain -- Growth -- Laboratory manuals; Electronic books
• Languages: English
• ISBNs: 9781493997329; 1493997327
• Related ISBNs: 9781493997312
• Notes: Note: Online resource; title from PDF title page (SpringerLink, viewed October 1, 2019).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.