Transgenic mouse : methods and protocols /: methods and protocols. ([2020])

• Record Type: Book
• Further Information: Note: Edited by Melissa A. Larson.
• Editors: Larson, Melissa A
• Contents: Preface… Table of Contents… Contributing Authors… 1. The History of Transgenesis Thomas L. Saunders 2. Pronuclear Microinjection of One-Cell Embryos Melissa A. Larson 3. Integrase-Mediated Targeted Transgenics through Pronuclear Microinjection Ruby Yanru Chen-Tsai 4. In Vivo Validation of CRISPR Reagents in Pre-Implantation Mouse Embryos Melissa A. Larson, Katelin A. Gibson, and Jay L. Vivian 5. Targeted Mutations in the Mouse via Embryonic Stem Cells Marina Gertsenstein, Joffrey Mianné, Lydia Teboul, and Lauryl M.J. Nutter 6. Blastocyst Microinjection with Embryonic Stem Cells Melissa A. Larson 7. Generation of Large Fragment Knock-In Mouse Models by Microinjecting into 2-Cell Stage Embryos Bin Gu, Marina Gertsenstein, and Eszter Posfai 8. Embryo Transfer Surgery Melissa A. Larson 9. Non-Surgical Embryo Transfer Protocol for use with the NSET™ Device Barbara J. Stone 10. Nuclear Transfer and Cloning Ling Liu 11. Sperm-Mediated Genetic Modifications Marialuisa Lavitrano, Laura Farina, Maria Grazia Cerrito, and Roberto Giovannoni 12. Genotyping Genetically Modified (GM) Mice Neeraj K. Aryal and Jan Parker-Thornburg 13. Nomenclature: Naming Your Gene-Modified Mouse Alicia Valenzuela 14. Breeding Strategies for Genetically Modified Mice Jan Parker-Thornburg 15. Strategies for Behaviorally Phenotyping the Transgenic Mouse Kenneth E. McCarson 16. Cryobanking and Recovery of Genetically Modified Mice Toru Takeo and Naomi Nakagata 17. Simple Transportation of Genetically Engineered Mice via Cold Storage Techniques Hidetaka Yoshimoto, Toru Takeo, and Naomi Nakagata 18. Reprogramming of Primary Human Cells to Induced Pluripotent Stem Cells using Sendai Virus Julia M. Draper and Jay L. Vivian.
• Publisher Details: New York : Humana Press
• Publication Date: 2020
• Copyright Date: 2020
• Extent: 1 online resource, illustrations (some color)
• Subjects: 616.02/73; Transgenic mice; Mice -- Genetic engineering; Mice as laboratory animals; Electronic books
• Languages: English
• ISBNs: 9781493998371; 1493998374
• Related ISBNs: 9781493998364
• Notes: Note: Online resource; title from PDF title page (SpringerLink, viewed September 24, 2019).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.