Electroporation protocols : microorganism, mammalian system, and nanodevice /: microorganism, mammalian system, and nanodevice. (2020)

• Record Type: Book
• Further Information: Note: Edited by Shulin Li, Lingqian Chang, Justin Teissie.
• Editors: Li, Shulin, 1961-; Chang, Lingqian; Teissie, Justin, 1947-
• Contents: Part I: Design and Fabrication of Micro-/Nano-Electroporation 1. Cell Size-specific Transfection by Micropillar Array Electroporation Xuan Liu, Yingbo Zu, and Shengnian Wang 2. Soft Electroporation through 3D Hollow Nanoelectrodes Jun Yin and Yang Li 3. Micromachining of Polymeric Microfluidic Micro-/Nano-Electroporation Device Lei Li 4. 3D Nanochannel Array for High-Throughput Cell Manipulation and Electroporation Lingqian Chang, Stephen Black, Chandani Chitrakar, and Mehdi Nouri 5. Low-Voltage Flow-Through Electroporation Membrane and Method Juliette Experton, Aaron G. Wilson, and Charles R. Martin Section II: Micro-/Nano-electroporation for in vitro Delivery 6. Nanofountain Probe Electroporation for Monoclonal Cell Line Generation Horacio D. Espinosa, Prithvijit Mukherjee, and Cesar Patino 7. 3D Nanochannel Electroporation for Macromolecular Nucleotides Delivery Lingqian Chang, Chandani Chitrakar, and Mehdi Nouri 8. Nanoelectroporation and Collection of Genetically Modified Exosomes in Primary Cultures of Dendritic Cells Silvia Duarte-Sanmiguel, Natalia Higuita-Castro, and Daniel Gallego-Perez 9. Electroporation of CRISPR-Cas9 into Malignant B Cells for Loss-of-function Studies of Target Gene via Knockout Jian Yu 10. Microfluidic Device for Localized Electroporation Justin Brooks and Ruiguo Yang Part III: Advanced in vivo Electroporation 11. Transdermal Delivery of Nucleic Acid Mediated by Punching and Electroporation Dong Huang, Yuanyu Huang, and Zhihong Li 12. Targeted in vivo Electroporation Using Nanoengineered Microelectrodes Daniel Schwarz and Andreas T. Schaefer 13. Zygote Electroporation for CRISPR/Cas9 Delivery to Generate Genetically Modified Mice Tatsuya Takemoto 14. Genome Editing in Staphylococcus aureus by Conditional Recombineering and CRISPR/Cas9-mediated Counterselection Kelsi Penewit and Stephen J. Salipante 15. Tissue Nano-Transfection of Surgically Exposed Stroma Natalia Higuita-Castro, Jordan Moore, Ludmila Diaz-Starokozheva, Cameron Rink, Ian Valerio, and Daniel Gallego-Perez Section IV: Microorganism Electroporation 16. Transformation of a Model Microalga, Chlamydomonas reinhardtii without Cell-wall Removal Takashi Yamano and Hideya Fukuzawa 17. Electroporation Transformation Protocol for Phaeodactylum tricornutum Hanhua Hu and Yufang Pan 18. Efficient Transformation of the Diatoms Phaeodactylum tricornutum by Multi-pulse Electroporation Kentaro Ifuku and Dongyi Yan 19. Rapid and Effective Electroporation Protocol for Nannochloropsis oceanica Da-Wei Li, Srinivasan Balamurugan, Jian-Wei Zheng, Wei-Dong Yang, Jie-Sheng Liu, and Hong-Ye Li 20. Electroporation Procedures for Genetic Modification of Green Algae Chlorella spp. Liang Ji and Jianhua Fan 21. Electrotransformation of Saccharomyces cerevisiae Meilhoc E and Teissié J.
• Edition: Third edition
• Publisher Details: New York, NY : Humana Press
• Publication Date: 2020
• Extent: 1 online resource (xi, 196 pages), illustrations (some color)
• Subjects: 571.6028; Electroporation; Gene therapy; Mobile genetic elements; Transfection; Electronic books
• Languages: English
• ISBNs: 9781493997404; 1493997408
• Related ISBNs: 9781493997398
• Notes: Note: Online resource; title from PDF title page (SpringerLink, viewed September 17, 2019).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.