Oligonucleotide-based therapies : methods and protocols /: methods and protocols. (2019)

• Record Type: Book
• Further Information: Note: Edited by Olof Gissberg, Rula Zain, and Karin E. Lundin.
• Editors: Gissberg, Olof; Zain, Rula; Lundin, Karin E
• Contents: Part I: Reviews 1. Chemical Development of Therapeutic Oligonucleotides Karin E. Lundin, Olof Gissberg, C.I. Edvard Smith, and Rula Zain 2. Tissue-Specific Delivery of Oligonucleotides Xin Xia, Nicolette Pollock, Jiehua Zhou, and John Rossi Part II: Protocols and Strategies 3. Designing siRNA and Evaluating Its Effect on RNA Targets Using qPCR and Western Blot Linda Vidarsdottir, Oksana Goroshchuk, Iryna Kolosenko, and Caroline Palm-Apergi 4. Antisense Oligonucleotides for Splice Modulation: Assessing Splice Switching Efficacy Cristina S.J. Rocha 5. Assessing Oligonucleotide Binding to Double-Strand DNA Negin Mozafari and Tea Umek 6. Modelling and Simulation of Oligonucleotide Hybrids: Outlining a Strategy Lennart Nilsson and Alessandra Villa 7. Characterization of Peptide/Oligonucleotide Complexes Using Electron Microscopy, Dynamic Light Scattering, and Protease Resistance Assay Kärt Padari, Ly Porosk, Piret Arukuusk, and Margus Pooga 8. Click-Shielded and Targeted Lipopolyplexes Philipp Michael Klein and Ernst Wagner 9. Attachment of Peptides to Oligonucleotides on Solid Support Using Copper(I)-Catalyzed Huisgen 1, 3-Dipolar Cycloaddition Malgorzata Honcharenko, Dmytro Honcharenko, and Roger Stromberg 10. Characterizing Oligonucleotide Uptake in Cultured Cells: A Case Study Using AS1411 Aptamer Elsa M. Reyes-Reyes and Paula J. Bates 11. Measuring the Action of Oligonucleotide Therapeutics in the Lung at the Cell Type-Specific Level by Tissue Disruption and Cell Sorting (TDCS) Helen Graves, Steven Evans, Michael Fauler, Manfred Frick, and Sterghios A. Moschos 12. Delivery of Antisense Oligonucleotides Mediated by a Hydrogel System: In Vitro and In Vivo Application in the Context of Spinal Cord Injury Pedro M.D. Moreno, Teresa Rodrigues, Marília Torrado, Isabel F. Amaral, and Ana P. Pêgo 13. Evaluation of Cell-Penetrating Peptide Delivery of Antisense Oligonucleotides for Therapeutic Efficacy in Spinal Muscular Atrophy Suzan M. Hammond, Frank Abendroth, Michael J. Gait, and Matthew J.A. Wood 14. Injection and Infusion of Compounds to the Central Nervous System Margherita Zamboni 15. In Vitro Assessment of the Hepatotoxicity Potential of Therapeutic Oligonucleotides Sabine Sewing, Tanja Minz, and Franziska Boess 16. Identifying Suitable Target Regions and Analyzing Off-Target Effects Lykke Pedersen, Peter H. Hagedorn, and Troels Koch 17. RACE-SEQ and Population-Wide Polymorphism Susceptibility Testing for Endonucleolytically Active, RNA-Targeting Therapeutics Louise Usher, Pantazis I. Theotokis, and Sterghios A. Moschos 18. Studying the Biotransformation of Phosphorothioate-Containing Oligonucleotide Drugs by LC-MS Christophe Husser, Erich Koller, Andreas Brink, and Simone Schadt 19. ADME: Assessing Pharmacokinetic-Pharmacodynamic Parameters of Oligonucleotides Rasmus Jansson-Löfmark*, Christine Ahlström, and Peter Gennemark.
• Publisher Details: New York, NY : Humana Press
• Publication Date: 2019
• Extent: 1 online resource (xi, 344 pages), illustrations (some color)
• Subjects: 572.8/5; Oligonucleotides -- Therapeutic use -- Laboratory manuals; Electronic books
• Languages: English
• ISBNs: 9781493996704; 1493996703
• Related ISBNs: 9781493996698
• Notes: Note: Online resource; title from PDF title page (SpringerLink, viewed September 6, 2019).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.