High-throughput protein production and purification : methods and protocols /: methods and protocols. (2019)

• Record Type: Book
• Further Information: Note: Edited by Renaud Vincentelli.
• Editors: Vincentelli, Renaud
• Contents: Part 1 General Procedures 1) HTP cloning 2) HTP generation of truncation and mutation libraries 3) HTP protein production in Escherichia coli 4) HTP Production of Protein Complexes by Co-expression in Escherichia coli 5) HTP protein complex reconstitution in Escherichia coli 6) HTP protein production in alternative bacterial system 7) HTP protein production in Yeast 8) HTP protein production and protein complexes in Baculovirus 9) HTP protein production and protein complexes in Mammalian cells 10) HTP protein production in plants 11) HTP protein expression in bacterial Cell Free system 12) HTP protein expression in eukaryotic Cell Free system 13) HTP refolding of proteins 14) HTP production of binders 15) HTP production of antibodies 16) HTP Modification of strains for improved protein expression (CHO, HEK, E. coli) Part 2 Case studies 17) HTP production of membrane proteins 18) HTP membrane protein expression in bacterial Cell Free system 19) HTP production of kinases 20) HTP production of small cysteine-rich proteins (VENOMICS) 21) HTP production of transcription factors 22) HTP Protein production for drug discovery Part 3 HTP micro-characterizations of proteins 23) Analytical SEC- MALLS 24) Differential Scanning Fluorimetry 25) Native mass spectrometry 26) Protein-protein interaction (Pull-down/Hold-up) 27) Protein-DNA (SELEX) 28) Analytical Electron microscopy.
• Publisher Details: New York, NY : Humana Press
• Publication Date: 2019
• Extent: 1 online resource (xvi, 537 pages), illustrations (some color)
• Subjects: 572/.645; Proteins -- Synthesis -- Laboratory manuals; Combinatorial chemistry -- Laboratory manuals; Electronic books
• Languages: English
• ISBNs: 9781493996247
• Related ISBNs: 149399624X; 9781493996230
• Notes: Note: Online resource; title from PDF title page (SpringerLink, viewed August 1, 2019).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.