Vaccinia Virus : Methods and Protocols /: Methods and Protocols. (2019)

• Record Type: Book
• Further Information: Note: Jason Mercer.
• Editors: Mercer, Jason
• Contents: 1-Laboratory safety and vaccinia (Isaacs) 2-Preparation and purification of VACV MVs and EVs 3-CRISPR mediated poxvirus genome editing (Yaohe Wang) 4-Vaccinia and ribosome profiling (Yang) 5-Pox bioinformatics (Sobhy) 6-High-through Image analyses of infection 7-RNAi against VACV genome proteins 8-Construction of recombinant VACVs (transient expressors/tagged) 9-Transient complementation of deletions with stable expressing cell lines (Weibe) 10- Super-resolution imaging of poxviruses (Albrect/Gray) 11-Vaccinia virus RNA sequencing (Yang Z or Moss) 12-Vaccinia antibody production (Cohen) 13-HTS for VACV cell targets (Mercer) 14-Drug screening against poxviruses (Hruby) 15-VACV DNA preparation for molecular biology cloning (Roper) 16-Transient complementation assays for mutant protein assessment (traktman) 17-VACV in vitro transcription from purified cores (broyles) 18-Studying vaccinia replication (Traktman) 19-Quatitaive PCR assessment of VACV genome copies (Moss) 20-Click-chemistry and genome labeling (Kilcher/Mok) 21-Vaccinia infection mouse models (buller) 22-Fluoresent recombinant VACV and live cell imaging (Newsome) 23-VACV bioluminescence imaging 24-VACV in vivo intravital imaging (Hickman Yewdell) 25-Pox and oncolytics (Mcfadden).
• Publisher Details: New York : Humana
• Publication Date: 2019
• Copyright Date: 2019
• Extent: 1 online resource (315 pages)
• Subjects: Medicine; Medical virology; Medical -- Microbiology; Medical microbiology & virology
• Languages: English
• ISBNs: 9781493995936
• Related ISBNs: 9781493995929
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.