Low-Cost Methods for Molecular Characterization of Mutant Plants : Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols /: Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols. (2015)

• Record Type: Book
• Further Information: Note: Bradley J. Till, Joanna Jankowicz-Cieslak, Owen A. Huynh, Mayada M. Beshir, Robert G. Laport, Bernhard J. Hofinger.
• Authors: Till, Bradley J; Jankowicz-Cieslak, Joanna; Huynh, Owen A; Beshir, Mayada M; Laport, Robert G; Hofinger, Bernhard J
• Contents: 1 Introduction1.1 Background1.2 Methods used to isolate genomic DNA from plant tissues1.3 Methods for the discovery and characterization of induced and natural nucleotide variation in plant genomesReferences2 Health and Safety Considerations 2.1 Guidelines 2.2 Preparation of a home-made chemical spill kitReferences3 Sample Collection and Storage3.1 Background3.2 Materials3.3 Methods References4 Low-Cost DNA Extraction 4.1 Materials 4.2 Methods 4.3 Alternative buffers for DNA extraction References5 PCR Amplification for Low-Cost Mutation Discovery 5.1 Materials 5.2 Methods References6 Enzymatic Mismatch Cleavage and Agarose Gel Evaluation of Samples6.1 Materials6.2 MethodsReferences7 Alternative Enzymology for Mismatch Cleavage for TILLING and Ecotilling: Extraction of Enzymes form Common Weedy Plants7.1 Materials7.2 Methods8 Example Data8.1 Quality of genomic DNA obtained by silica powder-based DNA extraction method8.2 Quality of genomic DNA obtained by silica powder-based DNA extraction using alternative buffers method8.3 Example of PCR products using TILLING primers with source genomic DNA from Qiagen kits and low-cost silica method8.4 Example of low-cost agarose gel based TILLING assays for the discovery of induced point mutations8.5 Example of enzyme activity recorded from weeds compared to crude celery juice extract9 Conclusions .
• Edition: 2015
• Publisher Details: Cham : Springer
• Publication Date: 2015
• Copyright Date: 2015
• Extent: 1 online resource (35 pages)
• Subjects: Life sciences; Plant breeding; Cytology -- Research -- Methodology; Nucleic acids; Science -- Life Sciences -- Biology -- General; Science -- Life Sciences -- Biochemistry; Biology, life sciences; Biochemistry; Science -- Life Sciences -- Botany; Plant reproduction & propagation
• Languages: English
• ISBNs: 9783319162591
• Related ISBNs: 9783319162584
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.