Antiviral Resistance in Plants : Methods and Protocols /: Methods and Protocols. (2019)

• Record Type: Book
• Further Information: Note: Kappei Kobayashi, Masamichi Nishiguchi.
• Editors: Kobayashi, Kappei; Nishiguchi, Masamichi
• Contents: Section 1. Analysis of natural virus resistance mechanisms 1 Identification and functional analysis of NB-LRR-type virus resistance genes Overview and Functional analysis of candidate genes 2-4 Analysis of resistance signaling pathways and resistance mechanisms 2 Genetic analysis in Arabidopsis thaliana 3 VIGS analysis in Nicotiana benthamiana 4 Resistance mechanisms 5 Identification and functional analysis of recessive virus resistance genes Overview and analytical methods 6-7 Identification of non-conventional virus resistance genes 6 Screening in diverse plants 7 Biochemical identification of virus resistance genes 8-9 Isolation and analysis of virus resistance breaking strains 8 Analysis of field isolates and Error-prone PCR-based mutagenesis 9 Forced evolution in plants and selection in grafted scions 10-12 Analysis of RNA silencing-mediated virus resistance 10 Analysis of small RNAs (small RNA gel blotting, Deep sequencing) 11 Analysis of DNA methylation and histone modification in virus resistance 12 Functional analysis of RNA silencing machinery Section 2. Engineered virus resistance and Virus vaccine: Design and Evaluation 13 RNA silencing-based virus resistance with viral RNA and DNA targets 14 Virus resistance by CRISPR/Cas9 targeting viral sequences 15 Resistance breeding through the control of host factors involved in virus replication 16 Virus resistance breeding through CRISPR/Cas9-mediated genome editing 17 Attenuated viruses: Isolation, evaluation and mechanisms 18 RNA silencing-mediated virus vaccine.
• Publisher Details: New York : Humana
• Publication Date: 2019
• Copyright Date: 2019
• Extent: 1 online resource (292 pages)
• Subjects: Life sciences; Botany; Medical virology; Medical -- Microbiology; Medical microbiology & virology; Science -- Life Sciences -- Botany; Botany & plant sciences
• Languages: English
• ISBNs: 9781493996353
• Related ISBNs: 9781493996346
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.