NADPH oxidases : methods and protocols /: methods and protocols. (2019)

• Record Type: Book
• Further Information: Note: Edited by Ulla G. Knaus, Thomas L. Leto.
• Editors: Knaus, Ulla G; Leto, Tom
• Contents: Part I: The NADPH Oxidase Family: Overviews 1. Intersecting Stories of the Phagocyte NADPH Oxidase and Chronic Granulomatous Disease William M. Nauseef and Robert A. Clark 2. Mammalian NADPH Oxidases Hélène Buvelot, Vincent Jaquet, and Karl-Heinz Krause Part II: Biochemistry and Model Systems 3. Enhanced Immunoaffinity Purification of Human Neutrophil Flavocytochrome B for Structure Determination by Electron Microscopy Algirdas J. Jesaitis, Marcia Riesselman, Ross Taylor, and Susan Brumfield 4. Purification and Characterization of DUOX Peroxidase Homology Domains (PHDs) Jennifer L. Meitzler 5. A Close-Up View of the Impact of Arachidonic Acid on the Phagocyte NADPH Oxidase Tania Bizouarn, Hager Souabni, Xavier Serfaty, Aicha Bouraoui, Rawand Masoud, Gilda Karimi, Chantal Houée-Levin, and Laura Baciou 6. NOX5 Cell-Free Assay for the High-Throughput Screening of Small Molecules Fiona Augsburger, Delphine Rasti, Yves Cambet, and Vincent Jaquet 7. Spectroscopy of NOX Protein Family Members Yoko Nakano and William M. Nauseef 8. Soluble Regulatory Proteins for Activation of NOX Family NADPH Oxidases Hideki Sumimoto, Reiko Minakami, and Kei Miyano 9. Insights into the NOX NADPH Oxidases Using Heterologous Whole Cell Assays Mary C. Dinauer 10. The X-CGD PLB-985 Cell Model for NOX2 Structure-Function Analysis Sylvain Beaumel and Marie José Stasia 11. Functional Characterization of DUOX Enzymes in Reconstituted Cell Models Agnieszka Korzeniowska, Ágnes P. Donkó, Stanislas Morand, and Thomas L. Leto 12. Guidelines for the Detection of NADPH Oxidases by Immunoblot and RT-qPCR Becky A. Diebold, S. Garrett Wilder, Xavier De Deken, Jennifer L. Meitzler, James H. Doroshow, James W. McCoy, Yerun Zhu, and J. David Lambeth Part III: Detection of ROS 13. Methods for Detection of NOX-Derived Superoxide Radical Anion and Hydrogen Peroxide in Cells Fiona Augsburger, Aleksandra Filippova, and Vincent Jaquet 14. HPLC-Based Monitoring of Oxidation of Hydroethidine for Detection of NADPH Oxidase-Derived Superoxide Radical Anion Jacek Zielonka, Monika Zielonka, and Balaraman Kalyanaraman 15. Visualization of Intracellular Hydrogen Peroxide with the Genetically-Encoded Fluorescent Probe HyPer in NIH-3T3 Cells Yulia G. Ermakova, Nataliya M. Mishina, Carsten Schultz, and Vsevolod V. Belousov 16. Imaging Intracellular H2O2 with the Genetically-Encoded PerFRET and OxyFRET Probes Balázs Enyedi and Miklós Geiszt 17. Quantitative Imaging of Endogenous and Exogenous H2O2 Gradients in Live Zebrafish Larvae Mark Jelcic, Balázs Enyedi, and Philipp Niethammer 18. Kinetic Analysis of Phagosomal ROS Generation Sophie Dupré-Crochet, Marie Erard, and Oliver Nüβe 19. Imaging Intestinal ROS in Homeostatic Conditions Using L-012 Emer Conroy and Gabriella Aviello 20. Hydro-Cy3-Mediated Detection of Reactive Oxygen Species In Vitro and In Vivo Bejan J. Saeedi, Bindu Chandrasekharan, and Andrew S. Neish Part IV: Regulation of NADPH Oxidase 21. Phosphorylation of Gp91phox/NOX2 in Human Neutrophils Houssam Raad, Riad Arabi Derkawi, Asma Tlili, Sahra A. Belambri, Pham My-Chan Dang, and Jamel El-Benna 22. The Molecular Regulation and Functional Roles of NOX5 David J.R. Fulton 23. Using Synthetic Peptides for Exploring Protein-Protein Interactions in the Assembly of the NADPH Oxidase Complex Edgar Pick 24. Rational Design and Delivery of NOX-Inhibitory Peptides Eugenia Cifuentes-Pagano and Patrick J. Pagano 25. High-Throughput Screening of NOX Inhibitors Jacek Zielonka, Monika Zielonka, Gang Cheng, Micael Hardy, and Balaraman Kalyanaraman 26. Protein-Protein Interaction Assay to Analyze NOX4/p22phox Heterodimerization Sharon O'Neill and Ulla G. Knaus Part V: Function of NADPH Oxidase 27. Isolation of Redox Active Endosomes (Redoxosomes) and Assessment of NOX Activity Weam S. Shahin and John F. Engelhardt 28. Model Systems to Investigate NOX-Dependent Cell Migration and Invasiveness Howard E. Boudreau and Thomas L. Leto 29. NADPH Oxidases and Aging Models of Lung Fibrosis Karen Bernard and Victor J. Thannickal 30. Proteomic Methods to Evaluate NOX-Mediated Redox Signaling Christopher M. Dustin, Milena Hristova, Caspar Schiffers, and Albert van der Vliet 31. Neutrophil Extracellular Traps Balázs Rada Part VI: Genetic Disorders 32. Chronic Granulomatous Disease Dirk Roos 33. Diagnostic Testing for Chronic Granulomatous Disease Douglas B. Kuhns 34. Gastrointestinal Complications in Chronic Granulomatous Disease E. Liana Falcone and Steven M. Holland 35. Ex Vivo Models of Chronic Granulomatous Disease Julie Brault, Bénédicte Vigne, and Marie Jose Stasia 36. Gene Editing in Chronic Granulomatous Disease Colin L. Sweeney, Randall K. Merling, Suk See De Ravin, Uimook Choi, and Harry L. Malech 37. DUOX Defects and Their Roles in Congenital Hypothyroidism Xavier De Deken and Françoise Miot 38. NAPDH Oxidases in Inflammatory Bowel Disease Emily Stenke, Billy Bourke, and Ulla G. Knaus.
• Publisher Details: New York, NY : Humana Press
• Publication Date: 2019
• Extent: 1 online resource (xv, 718 pages), illustrations (some color)
• Subjects: 572/.791; Oxidases -- Laboratory manuals; Electronic books
• Languages: English
• ISBNs: 9781493994243; 1493994247
• Related ISBNs: 9781493994236
• Notes: Note: Online resource; title from PDF title page (SpringerLink, viewed July 3, 2019).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.