Antigen Processing : Methods and Protocols /: Methods and Protocols. (2019)

• Record Type: Book
• Further Information: Note: Peter van Endert.
• Editors: Van Endert, Peter
• Contents: Preface… Table of Contents… Contributing Authors… 1. Purification of Large Cytosolic Proteases for In Vitro Assays: 20S and 26S Proteasomes Stefan Tenzer and Hansjörg Schild 2. Analysis of Proteasome Generated Antigenic Peptides by Mass Spectrometry Kathrin Textoris-Taube, Ulrike Kuckelkorn, Christin Keller, and Peter-M. Kloetzel 3. Trimming of MHC Class I Ligands by ERAP Aminopeptidases Mirjana Weimershaus, Irini Evnouchidou, Peter van Endert, and Marlene Bouvier 4. Peptide Trimming for MHC Class I Presentation by Endoplasmic Reticulum Aminopeptidases Mirco Compagnone and Doriana Fruci 5. Testing the Impact of Protease Inhibitors in Antigen Presentation Assays Michael Basler and Marcus Groettrup 6. In Vitro Studies of MHC Class I Peptide Loading and Exchange Marlene Bouvier 7. Measuring Synthesis and Degradation of MHC Class I Molecules Simon J. Powis and Antony N. Antoniou 8. Biochemical Analysis of Naturally Processed Antigenic Peptides Presented by MHC Class I Molecules Takayuki Kanaseki and Nilabh Shastri 9. A SIINFEKL Based System to Measure MHC Class I Antigen Presentation Efficiency and Kinetics Devin Dersh, Jonathan W. Yewdell, and Jiajie Wei 10. Purification and Identification of Naturally Presented MHC Class I and II Ligands Annika Nelde, Daniel J. Kowalewski, and Stefan Stevanović 11. Identification of MHC Ligands and Establishing MHC Class I Peptide Motifs Michael Ghosh, Moreno Di Marco, and Stefan Stevanović 12. Quantitating MHC Class I Ligand Production and Presentation using TCR-Like Antibodies Brian P. Dolan 13. Identifying Antigens Recognized by Cytolytic T Lymphocytes on Tumors Nathalie Vigneron, Vincent Stroobant, Wenbin Ma, Alexandre Michaux, and Benoît J. Van den Eynde 14. A Flow Cytometry-Based Approach to Unravel Viral Interference with the MHC Class I Antigen Processing and Presentation Pathway Patrique Praest, Hendrik de Buhr, and Emmanuel J.H.J. Wiertz 15. Evaluating CD8+ T Cell Responses In Vitro François A. Lemonnier 16. Recombinant Poxviruses: Versatile Tools for Immunological Assays Katherine S. Forsyth, Mary M. Addison, and Laurence C. Eisenlohr 17. Monitoring the Intracellular Routing of Internalized Antigens by Immunofluorescence Microscopy Maria Embgenbroich, Jasmin Mertins, Thorsten Lang, and Sven Burgdorf 18. Monitoring MHC Ubiquitination by MARCH Ubiquitin Ligases Evelina Gatti 19. Monitoring MHC-II Endocytosis and Recycling using Cell-Surface Protein Biotinylation-Based Assays Kyung-Jin Cho and Paul A. Roche 20. Monitoring Protein Endocytosis and Recycling using FACS-Based Assays Even Walseng and Paul A. Roche 21. In Vitro Digestion with Proteases Producing MHC Class II Ligands Mira Tohmé, Sophia Maschalidi, and Bénédicte Manoury 22. Assembly, Intracellular Transport and Release of MHC Class II Peptide Receptors Sebastian Temme, Nadine Temme, and Norbert Koch 23. Pulse-Chase Analysis for Studies of MHC Class II Biosynthesis, Maturation, and Peptide Loading Tieying Hou, Cornelia Rinderknecht, Debopam Ghosh, Andreas V Hadjinicolaou, Robert Busch, and Elizabeth D. Mellins 24. Studying MHC Class II Peptide Loading and Editing In Vitro AeRyon Kim, Isabel Ishizuka, Isamu Hartman, Yuri Poluektov, Kedar Narayan, and Scheherazade Sadegh-Nasseri 25. Monitoring Antigen Processing for MHC Presentation via Macroautophagy Monique Gannage, Rosa Barreira da Silva, and Christian Münz 26. Identification of Human Antigen-Specific CD4+ T-Cells with Peptide-MHC Multimer Technologies Ying Ying Kong and William W. Kwok 27. Production of Antigen Specific Human CD4+ T Cell Lines and Clones Jessica Matthis, Victoria King, and Helena Reijonen 28. Monitoring Dendritic Cell Activation and Maturation Christophe Macri, Ben Fancke, Kristen J. Radford, and Meredith O'Keeffe 29. Studying MHC Class II Presentation of Immobilized Antigen by B Lymphocytes Juan José Sáez, Ana-Maria Lennon-Duménil, and Maria-Isabel Yuseff 30. Analyzing Antigen Recognition by Natural Killer T Cells Sebastian Zeissig and Richard S. Blumberg.
• Edition: Second edition
• Publisher Details: New York : Humana
• Publication Date: 2019
• Copyright Date: 2019
• Extent: 1 online resource (458 pages)
• Subjects: Medicine; Monoclonal antibodies; Medical -- Immunology; Immunology; Science -- Life Sciences -- Biology -- Molecular Biology; Medical research
• Languages: English
• ISBNs: 9781493994502
• Related ISBNs: 9781493994496
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.