DNA repair : methods and protocols /: methods and protocols. (2019)

• Record Type: Book
• Further Information: Note: Edited by Lata Balakrishnan, Jason A. Stewart.
• Editors: Balakrishnan, Lata; Stewart, Jason A
• Contents: Preface…Table of Contents…Contributing Authors… Part I Reviews 1. Homologous Recombination-Mediated DNA Repair and Implications for Clinical Treatment of Repair Defective Cancers Nicole M. Reilly, Brian D. Yard, and Douglas L. Pittman 2. Resolving Roadblocks to Telomere ReplicationEmily Mason-Osann, Himabindu Gali, and Rachel L. Flynn Part II Cellular Assays to Detect and Measure DNA Damage and Damage Response 3. In Time and Space: Laser Micro-Irradiation and the DNA Damage ResponseJae Jin Kim, Ramhari Kumbhar, Fade Gong, and Kyle M. Miller 4. Quantification of Double Strand Breaks in Mammalian Cells using Pulsed-Field ElectrophoresisKelvin W. Pond and Nathan A. Ellis 5. Methods to Study Trinucleotide Repeat Instability Induced by DNA Damage and RepairYanhao Lai, Ruipeng Lei, Yaou Ren, and Yuan Liu 6. Extracting and Measuring dNTP Pools in Saccharomyces cerevisiaeRadha Subramaniam, Natalie A. Lamb, Yoonchan Hwang, Lauren Johengen, and Jennifer A. Surtees Part III Cellular and Cell Extract-Based Assays to Measure DNA Repair 7. Conversion Tract Analysis of Homology-Directed Genome Editing using Oligonucleotide DonorsYinan Kan and Eric A. Hendrickson 8. Reporter Assays for BER Pathways Dorota Piekna-Przybylska 9. Methods for Studying DNA Single-Strand Break Repair and Signaling in Xenopus laevis Egg ExtractsYunfeng Lin, Anh Ha, and Shan Yan 10. Chromatin Immunoprecipitation (ChIP) of Plasmid-Bound Proteins in Xenopus Egg ExtractsKelly B. Wolfe and David T. Long 11. Cellular Assays to Study the Functional Importance of Human DNA Repair HelicasesSanket Awate, Srijita Dhar, Joshua A. Sommers, and Robert M. Brosh, Jr. 12. A Mammalian Genetic Complementation Assay for Assessing Cellular Resistance to Genotoxic CompoundsNicole M. Reilly and Douglas L. Pittman 13. Small Molecule Inhibitor Screen for DNA Repair ProteinsJohn J. Turchi and Pamela S. VanderVere-Carozza Part IV In Vitro Biochemical and Biophysical Methods to Study DNA Repair 14. Assembling the Human Resectosome on DNA CurtainsMicheal M. Soniat, Logan R. Myler, and Ilya J. Finkelstein 15. Thin-Layer Chromatography and Real-Time Coupled Assays to Measure ATP HydrolysisChristopher W. Sausen, Cody M. Rogers, and Mathew L. Bochman 16. Gel-Based Assays for Measuring DNA Unwinding, Annealing, and Strand ExchangeCody M. Rogers, Christopher W. Sausen, and Matthew L. Bochman 17. In Vitro Assay for Plasmid Length DNA Strand Exchange by Human DMC1Steven D. Goodson, Russell B. Hawes, Sarah M. Waldvogel, and Michael G. Sehorn 18. In Vitro Assays for DNA Branch MigrationAndrew A. Kelso, Steven D. Goodson, and Michael G. Sehorn 19. Stabilization of Human DMC1 Nucleoprotein FilamentSarah M. Waldvogel, Steven D. Goodson, and Michael G. Sehorn Part V Methods to Assess Telomeric and Replication-Induced DNA Damage and Repair 20. Measuring UV Photoproduct Repair in Isolated Telomeres and Bulk Genomic DNA Elise Fouquerel, Ryan P. Barnes, Hong Wang, and Patricia L. Opresko 21. Single-Molecule DNA Fiber Analyses to Characterize Replication Fork Dynamics in Living Cells Srijita Dhar, Arindam Datta, Taraswi Banerjee, and Robert M. Brosh Jr. 22. Direct Visualization of DNA Replication at Telomeres using DNA Fiber Combing Combined with Telomere FISHHimabindu Gali, Emily Mason-Osann, Rachel Litman Flynn 23. Telomere and G-Quadruplex Co-Localization Analysis by Immunofluorescence-Fluorescence In Situ Hybridization (IF-FISH)Miaomiao Zhang, Rui Lui, and Feng Wang 24. FISHing for Damage on Metaphase ChromosomesP. Logan Schuck, Jason A. Stewart.
• Publisher Details: New York, NY : Humana Press
• Publication Date: 2019
• Extent: 1 online resource (xv, 352 pages), illustrations (some color)
• Subjects: 572.8/6459; DNA repair -- Laboratory manuals; Electronic books
• Languages: English
• ISBNs: 9781493995004; 1493995006
• Related ISBNs: 9781493994991
• Notes: Note: Online resource; title from PDF title page (SpringerLink, viewed June 21, 2019).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.