One-step generation of a drug-releasing microarray for high-throughput small-volume bioassays. (c2019)

• Record Type: Book
• Further Information: Note: Seo Woo Song.
• Other Names: Song, Seo Woo
• Contents: Intro; Supervisor's Foreword; Abstract; Parts of this thesis have been published in the following documents:; Acknowledgements; Contents; List of Figures; List of Tables; 1 Introduction; 1.1 High-Throughput Small-Volume Bioassays; 1.1.1 Miniaturization Trends in Biochemical Screening Platform; 1.1.2 Clinical Value of the Small-Volume HTS Platform; 1.1.3 Lab-on-a-Chip Based HTS Platforms; 1.2 Developmental Goal for the 'Pipetting-Free' HTS Platforms; 1.2.1 Difficulties in Automation of Liquid Handling System; 1.2.2 Previous Researches for 'Pipetting-Free' HTS Platforms from Other Groups 1.3 Main Concept: One-Step Generation of a Drug-Releasing Microarray-on-a-Chip by Self-assembly of Drug-Laden Microparticles (DLPs)1.3.1 Previous Works of Partipetting from Our Group and Their Limitations; 1.3.2 My Works in This Dissertation; References; 2 System Development; 2.1 Sealing-Film Assisted Seeding Method for Saving Cell Consumptions; 2.2 Chip and Jig Development; 2.2.1 Polystyrene-Poly(Dimethylsiloxane) (PS-PDMS) Hybrid Chip for Precise Alignment and Sealing; 2.3 Preparation of DLPs Library; 2.3.1 Microparticles as Drug Carriers and Requirements for Drug Loading 2.3.2 Strategies to Increase the Absorbing Amount of Drugs into Hydrogel Microparticles (Failed Trial 1)2.3.3 Mixing Drug Solution with Prepolymer to Fabricate Microparticles (Failed Trial 2); 2.3.4 Drug Loading into Prefabricated Microparticles by Freeze-Drying; 2.4 Decoding Microparticles; 2.4.1 Design of Graphical Codes on the Microparticles; 2.4.2 Decoding by Neural-Network-Based Recognition of Coding Components; 2.4.3 Neural-Network-Based Decoding from an Image of a Whole Microparticle; 2.5 Statistical Analysis for Duplications 2.5.1 Binomial Distribution Model for Random Assembly of Microparticles2.5.2 Monte-Carlo Simulation for Statistical Analysis; References; 3 Application: Screening of Sequential Drug Combinations; 3.1 Therapeutic Benefit of Sequential Drug Combination Based on Rewiring of Intracellular Pathways; 3.2 Screening of Sequential Drug Combination Using a Partipetting Platform; 3.3 Proof-of-Concept: Sequential Combinatorial Cell Staining Assay by Replacement of the Drug Chip 3.4 Screening of Sequential Combinatorial Drugs with EGFR Inhibitor Followed by Genotoxin Against Triple Negative Breast Cancer (TNBC)References; 4 Conclusion and Discussion
• Publisher Details: Singapore : Springer
• Publication Date: 2019
• Copyright Date: 2019
• Extent: 1 online resource (71 p.)
• Subjects: 615.7/04; Drugs -- Testing; Biological assay; Electronic books; Electronic books
• Languages: English
• ISBNs: 9789811380945; 9811380945
• Related ISBNs: 9811380937; 9789811380938
• Notes: Note: Description based upon print version of record.
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.