Single cell methods : sequencing and proteomics /: sequencing and proteomics. (2019)
- Record Type:
- Book
- Title:
- Single cell methods : sequencing and proteomics /: sequencing and proteomics. (2019)
- Main Title:
- Single cell methods : sequencing and proteomics
- Further Information:
- Note: Edited by Valentina Proserpio.
- Editors:
- Proserpio, Valentina
- Contents:
- Acknowledgement…Preface…Table of Contents…Contributing Authors… Part I Lab Setup and Tissue Preparation 1. Setting Up a Single-Cell Genomic LaboratoryLira Mamanova 2. Tissue Handling and Dissociation for Single-Cell RNA-seqFelipe Vieira Braga and Ricardo J. Miragaia Part II Single Cell Trancriptomic Analysis 3. Full-Length Single-Cell RNA-Sequencing with Smart-seq2Simone Picelli 4. CEL-Seq2 – Single Cell RNA Sequencing by Multiplexed Linear Amplification Itai Yanai and Tamar Hashimshony 5. Single-Cell RNA-seq by Multiple Annealing and Tailing Based Quantitative Single-Cell RNA-seq (MATQ-seq)Kuanwei Sheng and Chenghang Zong 6. Single-Cell RNA Sequencing with Drop-SeqJosephine Bageritz and Gianmarco Raddi 7. Chromium 10X Single Cell 3' mRNA Sequencing of Tumor Infiltrating Lymphocytes Marco De Simone, Grazisa Rossetti, and Massimiliano Pagani 8. Seq-Well: A Sample-Efficient, Portable Picowell Platform for Massively Parallel Single-Cell RNA-SequencingToby P. Aicher, Shaina Carroll, Gianmarco Raddi, Todd Gierahn, Marc H. Wadsworth II, Travis K. Hughes, Chris Love, and Alex K. Shalek 9. Single-Cell Tagged Reverse Transcription (STRT-seq)Kedar Natarajan 10. Single-Cell RNA-Sequencing of Peripheral Blood Mononuclear Cells with ddSEQShaheen Khan and Kelly A. Kaihara 11. High Throughput Single Cell Real-Time Quantitative PCR AnalysisLiora Haim-Vilmovsky 12. Single-Cell Dosing and mRNA Sequencing of Suspension and Adherent Cells Using the Polaris™ SystemChad D. Sanada and Aik T. OoiAcknowledgement…Preface…Table of Contents…Contributing Authors… Part I Lab Setup and Tissue Preparation 1. Setting Up a Single-Cell Genomic LaboratoryLira Mamanova 2. Tissue Handling and Dissociation for Single-Cell RNA-seqFelipe Vieira Braga and Ricardo J. Miragaia Part II Single Cell Trancriptomic Analysis 3. Full-Length Single-Cell RNA-Sequencing with Smart-seq2Simone Picelli 4. CEL-Seq2 – Single Cell RNA Sequencing by Multiplexed Linear Amplification Itai Yanai and Tamar Hashimshony 5. Single-Cell RNA-seq by Multiple Annealing and Tailing Based Quantitative Single-Cell RNA-seq (MATQ-seq)Kuanwei Sheng and Chenghang Zong 6. Single-Cell RNA Sequencing with Drop-SeqJosephine Bageritz and Gianmarco Raddi 7. Chromium 10X Single Cell 3' mRNA Sequencing of Tumor Infiltrating Lymphocytes Marco De Simone, Grazisa Rossetti, and Massimiliano Pagani 8. Seq-Well: A Sample-Efficient, Portable Picowell Platform for Massively Parallel Single-Cell RNA-SequencingToby P. Aicher, Shaina Carroll, Gianmarco Raddi, Todd Gierahn, Marc H. Wadsworth II, Travis K. Hughes, Chris Love, and Alex K. Shalek 9. Single-Cell Tagged Reverse Transcription (STRT-seq)Kedar Natarajan 10. Single-Cell RNA-Sequencing of Peripheral Blood Mononuclear Cells with ddSEQShaheen Khan and Kelly A. Kaihara 11. High Throughput Single Cell Real-Time Quantitative PCR AnalysisLiora Haim-Vilmovsky 12. Single-Cell Dosing and mRNA Sequencing of Suspension and Adherent Cells Using the Polaris™ SystemChad D. Sanada and Aik T. Ooi 13. Targeted TCR Amplification from Single-Cell cDNA LibrariesShuqiang Li and Kenneth J. Livak Part III Single Cell Genomic and Epigenomic Analysis 14. Sequencing the Genomes of Single CellsVeronica Gonzalez-Pena and Charles Gawad 15. Studying DNA Methylation in Single Cell Format with scBS-seqNatalia Kunowska 16. Single Cell 5fC SequencingChenxu Zhu, Yun Gao, Jinying Peng, Fuchou Tang, and Chengqi Yi 17. ChIPmentation for Low-Input Profiling of In Vivo Protein-DNA InteractionsNatalia Kunowska and Xi Chen Part IV Single Cell Proteomic Analysis 18. Immuno-Phenotyping of Human Peripheral Blood Mononuclear Cells by Mass CytometrySusanne Heck, Cynthia Jane Bishop, and Richard Jonathan Ellis 19. Classification of the Immune Composition in the Tumor InfiltrateDavide Brusa and Jean-Luc Balligand Part V Single Cell Multi Omic Analysis 20. Combined Genome and Transcriptome (G&T) Sequencing of Single Cells Iraad F. Bronner and Stephan Lorenz 21. Simultaneous Pofiling of mRNA Transcriptome and DNA Methylome from a Single CellYoujin Hu, Qin An, Ying Guo, Jiawei Zhong, Shuxin Fan, Pinhong Rao, Xialin Liu, Yizhi Liu, and Guoping Fan 22. Simultaneous Targeted Detection of Proteins and RNAs in Single CellsAik T. Ooi and David W. Ruff Part VI Single Cell Screening 23. CRISPR-Screening in Single Cells Johan Henriksson Part VII Single Cell Live Imaging 24. Single Cell Live ImagingToru Hiratsuka and Naoki Komatsu Part VIII Single Cell Data Analysis 25. Differential Expression Analysis in Single Cell TranscriptomicsLuca Alessandri, Maddalena Arigoni, and Raffaele Calogero 26. A Bioinformatic Toolkit for Single-Cell mRNA AnalysisKevin Baßler, Patrick Günther, Jonas Schulte-Schrepping, Matthias Becker, and Paweł Biernat. … (more)
- Publisher Details:
- New York, NY : Humana Press
- Publication Date:
- 2019
- Extent:
- 1 online resource (xvi, 459 pages), illustrations (some color)
- Subjects:
- 571.6
Cytology -- Laboratory manuals
Electronic books - Languages:
- English
- ISBNs:
- 9781493992409
1493992406 - Related ISBNs:
- 9781493992393
- Notes:
- Note: Online resource; title from PDF title page (SpringerLink, viewed May 8, 2019).
- Access Rights:
- Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
- Access Usage:
- Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.
- View Content:
- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library HMNTS - ELD.DS.416478
- Ingest File:
- 02_521.xml