DNA cloning a hands-on approach /: a hands-on approach. (2019)

• Record Type: Book
• Further Information: Note: Seok-Yong Choi, Hyunju Ro, Hankuil Yi.
• Other Names: Choi, Seok-Yong; Ro, Hyunju; Yi, Hankuil
• Contents: Part I. What is cloning? -- Chapter 1.1 Definition of cloning -- Chapter 1.2 Discovering a new gene -- Chapter 1.3 Cloning in the past -- Chapter 1.4 Cloning in the present -- Part II. A prerequisite for cloning -- Chapter 2.1 Software useful for cloning design -- Chapter 2.2 Vector, plasmid, construct and the Kozak consensus sequence -- Chapter 2.3 Multiple cloning sites (MCS) -- Chapter 2.4 Restriction endonucleases and star activity -- Chapter 2.5 Agarose gel electrophoresis -- Chapter 2.6 Pouring LB agar plates -- Chapter 2.7 Competent cells -- Chapter 2.8 The conversion of DNA mass into molar concentration -- Chapter 2.9 Upon receiving new plasmids -- Chapter 2.10 cDNA library -- Part III. The first step in cloning -- Chapter 3.1 Cut & paste -- Chapter 3.2 DNA sequencing and direct sequencing -- Chapter 3.3 PCR and nested PCR -- Chapter 3.4 Fill-in (Full & Partial) -- Chapter 3.5 Compatible cohesive ends -- Chapter 3.6 Methylation -- Chapter 3.7 Three-piece ligation -- Chapter 3.8 Site-directed mutagenesis -- Chapter 3.9 Structure of plant transformation vectors -- Chapter 3.10 Transformation of R. radiobacter -- Part IV. The next step of cloning -- Chapter 4.1 How to insert a DNA fragment into a gene -- Chapter 4.2 How to delete an internal region of a gene -- Chapter 4.3 How to insert an epitope tag into a gene -- Chapter 4.4 Translational fusion vs. transcriptional fusion -- Part V. The last steps of cloning -- Chapter 5.1 Method for cloning similar genes in different species -- Chapter 5.2 RACE (rapid amplification of cDNA ends) -- Chapter 5.3 BAC recombineering -- Chapter 5.4 Old trick: partial digestion -- Chapter 5.5 Modification of a vector -- Chapter 5.6 When you notice a frame shift mutation upon cloning -- Chapter 5.7 Reality of cloning: an extremely unlucky case -- Part VI. Methods that make your cloning life easier -- Chapter 6.1 TA cloning and production of a T-vector -- Chapter 6.2 TOPO TA cloning -- Chapter 6.3 Gateway cloning -- Chapter 6.4 Golden Gate Assembly for a modular cloning -- Chapter 6.5 In-Fusion Sequence and Ligation-Independent Cloning (In-Fusion SLIC) -- Chapter 6.6 T4 DNA Polymerase Sequence-and Ligation-Independent Cloning (T4 DNA Pol SLIC) -- Chapter 6.7 Non-template PCR cloning -- Part VII. Advice to cloners -- Chapter 7.1 When cloning is not going well -- Chapter 7.2 Keep your cloning data organized -- Appendix 1. Further readings -- Appendix 2. Abbreviations -- Appendix 3. Index.
• Publisher Details: Dordrecht : Springer
• Publication Date: 2019
• Extent: 1 online resource (139 p.)
• Subjects: 660.6/5; Molecular cloning; Clone cells; Gene Expression; Genetic Engineering; Plant Genetics and Genomics; Animal Genetics and Genomics; Electronic books
• Languages: English
• ISBNs: 9789402416626; 9402416625
• Related ISBNs: 9789402416602; 9402416609
• Notes: Note: Includes bibliographical references and index.
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.