Synthesis and biological evaluation of pyrrole -- imidazole polyamide probes for visualization of telomeres. (2019)
- Record Type:
- Book
- Title:
- Synthesis and biological evaluation of pyrrole -- imidazole polyamide probes for visualization of telomeres. (2019)
- Main Title:
- Synthesis and biological evaluation of pyrrole -- imidazole polyamide probes for visualization of telomeres
- Further Information:
- Note: Yusuke Kawamoto.
- Authors:
- Kawamoto, Yusuke
- Contents:
- Intro; Supervisor's Foreword; Parts of this thesis have been published in the following journal articles:; Acknowledgements; Contents; 1 Introduction: Sequence-Specific DNA Binding Pyrrole-Imidazole Polyamides and Their Applications; 1.1 Introduction; 1.2 Hairpin Structure and Pairing Rule of Py-Im Polyamides; 1.3 Varied Structures of Py-Im Polyamides; 1.4 Regulation of Gene Expression by Py-Im Polyamides; 1.5 Repression of Gene Expression by Sequence-Specific DNA Alkylation; 1.6 Transcriptional Activation; 1.7 Py-Im Polyamides for Imaging 1.8 Other Functions and Applications of Py-Im Polyamides1.9 Conclusion and Outlook; 1.10 Scope of the Work; References; 2 Development of a New Method for Synthesis of Tandem Hairpin Pyrrole-Imidazole Polyamide Probes Targeting Human Telomeres; 2.1 Introduction; 2.2 Results and Discussion; 2.2.1 Synthesis of Conjugates 1-4; 2.2.2 UV-Visible Absorption Spectra and Fluorescence Spectra; 2.2.3 Binding Affinities and Specificities of Tandem Hairpin PI Polyamides; 2.3 Conclusion; 2.4 Supporting Information; 2.4.1 Materials for the Synthesis 2.4.2 Materials for the Analysis of Spectra and Thermal Denaturation Analysis2.4.3 Synthetic Details of PI Polyamides; 2.4.4 Materials for Telomere Staining; 2.4.5 Telomeres Staining; References; 3 Tandem Trimer Pyrrole-Imidazole Polyamide Probes Targeting 18 Base Pairs in Human Telomere Sequences; 3.1 Introduction; 3.2 Results and Discussion; 3.2.1 Synthesis of Py-Im Polyamides; 3.2.2 Optical CharacteristicsIntro; Supervisor's Foreword; Parts of this thesis have been published in the following journal articles:; Acknowledgements; Contents; 1 Introduction: Sequence-Specific DNA Binding Pyrrole-Imidazole Polyamides and Their Applications; 1.1 Introduction; 1.2 Hairpin Structure and Pairing Rule of Py-Im Polyamides; 1.3 Varied Structures of Py-Im Polyamides; 1.4 Regulation of Gene Expression by Py-Im Polyamides; 1.5 Repression of Gene Expression by Sequence-Specific DNA Alkylation; 1.6 Transcriptional Activation; 1.7 Py-Im Polyamides for Imaging 1.8 Other Functions and Applications of Py-Im Polyamides1.9 Conclusion and Outlook; 1.10 Scope of the Work; References; 2 Development of a New Method for Synthesis of Tandem Hairpin Pyrrole-Imidazole Polyamide Probes Targeting Human Telomeres; 2.1 Introduction; 2.2 Results and Discussion; 2.2.1 Synthesis of Conjugates 1-4; 2.2.2 UV-Visible Absorption Spectra and Fluorescence Spectra; 2.2.3 Binding Affinities and Specificities of Tandem Hairpin PI Polyamides; 2.3 Conclusion; 2.4 Supporting Information; 2.4.1 Materials for the Synthesis 2.4.2 Materials for the Analysis of Spectra and Thermal Denaturation Analysis2.4.3 Synthetic Details of PI Polyamides; 2.4.4 Materials for Telomere Staining; 2.4.5 Telomeres Staining; References; 3 Tandem Trimer Pyrrole-Imidazole Polyamide Probes Targeting 18 Base Pairs in Human Telomere Sequences; 3.1 Introduction; 3.2 Results and Discussion; 3.2.1 Synthesis of Py-Im Polyamides; 3.2.2 Optical Characteristics of the Fluorescent Probes; 3.2.3 Binding Affinities to Match Sequence and Discrimination of Mismatch Sequences; 3.2.4 Human Telomere Staining with Two Py-Im Polyamide Probes 3.3 Conclusion3.4 Supporting Information; 3.4.1 Materials; 3.4.2 Synthesis of Py-Im Polyamides; 3.4.3 Absorption and Fluorescence Spectra; 3.4.4 SPR Assays; 3.4.5 Telomeres Staining; References; 4 Targeting 24 bp Within Telomere Repeat Sequences with Tandem Tetramer Pyrrole-Imidazole Polyamide Probes; 4.1 Introduction; 4.2 Results and Discussion; 4.2.1 Synthesis of Py-Im Polyamides; 4.2.2 Comparative Analysis of Binding to Match Sequence; 4.2.3 Visualization of Human Telomeres to Compare Py-Im Polyamide Probes 4.2.4 High-Throughput Sequencing of the Binding Sites of Py-Im Polyamide Probes for Telomeric Repeats4.3 Conclusion; 4.4 Supporting Information; 4.4.1 Materials; 4.4.2 Synthetic Details of Py-Im Polyamides; 4.4.3 SPR Assays; 4.4.4 Telomeres Staining and Calculation of S/B Ratios; 4.4.5 Identification of TDi59-A and TTet59-B Binding Sites in Genome; References; Curriculum Vitae … (more)
- Publisher Details:
- Singapore : Springer Nature Springer
- Publication Date:
- 2019
- Extent:
- 1 online resource
- Subjects:
- 547/.593
Pyrroles
Imidazoles
SCIENCE / Chemistry / Organic
Electronic books
Electronic books - Languages:
- English
- ISBNs:
- 9789811369124
9811369127 - Related ISBNs:
- 9789811369117
9811369119 - Notes:
- Note: Includes bibliographical references.
Note: Online resource; title from PDF title page (SpringerLink, viewed March 13, 2019). - Access Rights:
- Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
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- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library HMNTS - ELD.DS.411323
- Ingest File:
- 02_511.xml