Hydrocarbon and Lipid Microbiology Protocols : Microbial Quantitation, Community Profiling and Array Approaches /: Microbial Quantitation, Community Profiling and Array Approaches. (2016)

• Record Type: Book
• Further Information: Note: Terry J. McGenity, Kenneth N. Timmis, Balbina Nogales.
• Editors: McGenity, Terry J; Timmis, Kenneth N; Nogales, Balbina
• Contents: Introduction to Microbial Quantitation, Community Profiling, and Array Approaches.- A cell extraction method for oily sediments.- Introduction to microplate MPN-enumeration of hydrocarbon degraders.- Primers for dsr genes and Most Probable Number Method for detection of sulfate-reducing bacteria in oil reservoirs.- Microbial biomass and community analysis by PLFA analysis.- Real-time PCR approaches for analysis of hydrocarbon-degrading bacterial communities.- Clone libraries of ribosomal RNA gene sequences for characterization of microbial communities.- Denaturing gradient gel electrophoresis (DGGE) for microbial community analysis.- Microbial community profiling: SSCP and T-RFLP techniques.- Microbial community analysis by single-amplicon high-throughput sequencing (metagenetics) – preparation, data handling and ecological analysis.- Multiplex fluorescent antibody microarrays and antibody graphs for microbial and biomarker detection in the environment.- Studying protistan communities in hydrocarbon-contaminated environments.
• Publisher Details: Berlin, Heidelberg : Springer
• Publication Date: 2016
• Copyright Date: 2017
• Extent: 1 online resource (252 pages)
• Subjects: Life sciences; Microbiology; Biochemical engineering; Technology & Engineering -- Chemical & Biochemical; Biochemical engineering; Science -- Life Sciences -- Biology -- Microbiology; Microbiology (non-medical)
• Languages: English
• ISBNs: 9783662527788
• Related ISBNs: 9783662527764
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.