Brown adipose tissue. (2019)

• Record Type: Book
• Further Information: Note: Alexander Pfeifer, Martin Klingenspor, Stephan Herzig, editors.
• Editors: Pfeifer, Alexander; Klingenspor, Martin, 1961-; Herzig, Stephan
• Contents: Intro; Preface; Contents; Brown Adipose Tissue Development and Metabolism; 1 Introduction and Background; 1.1 Overview; 1.2 Basics of Non-shivering Thermogenesis; 1.3 Brown Fat Anatomy and Morphology; 1.4 BAT Vascularization and Innervation; 1.5 Transcriptional Control of Brown Adipocyte Differentiation; 2 Brown Fat Growth; 2.1 Techniques for Studying BAT Development; 2.1.1 FACS; 2.1.2 Lineage Tracing; 2.2 Brown Adipocyte Origins; 2.3 Postnatal and Adult Brown Fat Growth and Metabolism; 3 Other Thermogenic Adipocytes; 3.1 Brite/Beige Adipocytes; 3.2 Brite/Beige Adipocyte Origins 4 Going Forward4.1 Unanswered Questions and Future Goals; 4.2 Prospects for BAT-Based Therapeutics; References; Lessons from Cre-Mice and Indicator Mice; 1 Introduction; 2 Techniques to Create Transgenic Mouse Lines; 3 Tamoxifen Inducible vs. Dox-Inducible Recombinase Systems; 4 The ap2 Promoter; 5 The Adiponectin Promoter; 6 The Ucp1 Promoter; 7 Genetic Mouse Models to Disrupt Mature Brown Adipocyte Function; 8 Lessons from Fat but Non-brown Fat-Specific Gene Targeting and Tracing; 9 Indicator Mice of Brown Fat Function; 10 White/Brown/Brite Adipocyte-Specific Labeling; References Aging of Brown and Beige/Brite Adipose Tissue1 Introduction; 2 General Aspects of White and Brown Adipose Tissue Aging; 3 Molecular Characteristics of Brown Adipose Tissue Aging; 4 Cellular Senescence: Stem Cell Dysfunction; 5 Extracellular Matrix and Adipose Tissue Aging; 6 Neuronal Regulation of Aging Brown Adipocytes; 7 Age-Related Changes to the Endocrine Regulation of Brown Adipocytes; 8 Contribution of Inflammatory Processes to BAT Atrophy; 9 Conclusion; References; Adipogenesis in Primary Cell Culture; 1 Introduction; 2 Biopsy; 3 Proliferation; 3.1 Priming/Adipocyte Commitment 3.2 Flow Cytometry for Evaluation of Cell Culture Purity4 Confluence; 5 Initiation of Differentiation; 6 Differentiation; 7 Validation of Cell Cultures; 8 Considerations; 8.1 Cell Passaging; 8.2 Differentiation With or Without Serum; 8.3 Inducing Brown Adipogenesis with External Factors; References; In Vitro Models for Study of Brown Adipocyte Biology; 1 Introduction; 2 In Vitro Adipocyte Research Tools; 3 Commonly Used In Vitro Models for Studying Brown Adipocyte Biology; 3.1 Characteristics of Brown Adipocytes; 3.2 Brown Adipose Tissue Explant Culture; 3.3 Primary Culture 3.4 Brown Adipocyte Cell Lines3.4.1 T37i; 3.4.2 WT-1; 3.4.3 HIB 1B; 3.4.4 PAZ6; 4 Conditionally Immortalised Brown Adipocyte Cell Lines; 5 Self-Immortalised Adipocyte Cell Lines; 6 Mouse Embryo Fibroblasts; 7 Human Multipotent Adipose-Derived Stem Cells; 8 Pluripotent Stem Cells; 9 Conclusion; References; Brown-Like Adipocyte Progenitors Derived from Human iPS Cells: A New Tool for Anti-obesity Drug Discovery and Cell-Based Thera...; 1 Introduction; 2 Capacity of Human-Induced Pluripotent Stem Cells to Generate BAs; 3 Pathways Governing hiPSC-BAP Adipogenic Capacity
• Publisher Details: Cham, Switzerland : Springer
• Publication Date: 2019
• Extent: 1 online resource (viii, 424 pages), illustrations (some color)
• Subjects: 611/.018276; Brown adipose tissue; Electronic books; Electronic books
• Languages: English
• ISBNs: 9783030105136
• Related ISBNs: 303010513X; 9783030105129; 3030105121
• Notes: Note: Online resource; title from PDF title page (SpringerLink, viewed April 11, 2019).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.