Understanding light microscopy. (2019)
- Record Type:
- Book
- Title:
- Understanding light microscopy. (2019)
- Main Title:
- Understanding light microscopy
- Further Information:
- Note: Jeremy Sanderson.
- Authors:
- Sanderson, J. B (Jeremy B.)
- Contents:
- About the Author ix Acknowledgements xi Look-Up Guide to Feature Boxes by Theme xii Glossary and Definitions xiv Notes xxiv Introduction xxvii 1 Our Eyes and the Microscope 1 2 Light 29 3 Basic Microscope Optics 55 4 Microscope Anatomy and Design 75 5 Ergonomics 91 6 Optical Aberrations of the Microscope 101 7 The Microscope Objective 127 8 Condensers and Eyepieces 161 9 Illumination in the Microscope 177 10 Diffraction and Image Formation in Microscopy 211 11 Contrast Generation and Enhancement 243 12 Reflected-Light Microscopy 289 13 Polarised-Light Microscopy: Part 1 – Theory 317 14 Polarised-Light Microscopy: Part 2 – Applied 347 15 Fluorescence Microscopy 383 16 Fluorophores and Fluorescent Proteins 405 17 Optical Sectioning and Confocal Microscopy 425 18 Operating the Confocal Microscope 447 19 Light-Sheet Microscopy 483 20 Bleed-Through and Spectral Unmixing 507 21 Deconvolution 523 22 Multi-Photon Microscopy 543 23 Total Internal Reflection Fluorescence Microscopy 561 24 FRAP and FRET 569 25 Colocalisation 587 26 Super-Resolution Fluorescence Microscopy 613 27 Choosing a Microscope Platform and Core Imaging Facilities 637 28 Biological Specimen Preparation 663 29 Materials Specimen Preparation 687 30 Recording the Image: Part 1 – Theory 707 31 Recording the Image: Part 2 – Applied 733 Appendices 1 Buying, and Tendering for, a Light Microscope 769 2 Troubleshooting Poor Image Quality 773 3 The Michel-Lévy Interference Colour Chart 775 4 Cleaning and Maintenance of theAbout the Author ix Acknowledgements xi Look-Up Guide to Feature Boxes by Theme xii Glossary and Definitions xiv Notes xxiv Introduction xxvii 1 Our Eyes and the Microscope 1 2 Light 29 3 Basic Microscope Optics 55 4 Microscope Anatomy and Design 75 5 Ergonomics 91 6 Optical Aberrations of the Microscope 101 7 The Microscope Objective 127 8 Condensers and Eyepieces 161 9 Illumination in the Microscope 177 10 Diffraction and Image Formation in Microscopy 211 11 Contrast Generation and Enhancement 243 12 Reflected-Light Microscopy 289 13 Polarised-Light Microscopy: Part 1 – Theory 317 14 Polarised-Light Microscopy: Part 2 – Applied 347 15 Fluorescence Microscopy 383 16 Fluorophores and Fluorescent Proteins 405 17 Optical Sectioning and Confocal Microscopy 425 18 Operating the Confocal Microscope 447 19 Light-Sheet Microscopy 483 20 Bleed-Through and Spectral Unmixing 507 21 Deconvolution 523 22 Multi-Photon Microscopy 543 23 Total Internal Reflection Fluorescence Microscopy 561 24 FRAP and FRET 569 25 Colocalisation 587 26 Super-Resolution Fluorescence Microscopy 613 27 Choosing a Microscope Platform and Core Imaging Facilities 637 28 Biological Specimen Preparation 663 29 Materials Specimen Preparation 687 30 Recording the Image: Part 1 – Theory 707 31 Recording the Image: Part 2 – Applied 733 Appendices 1 Buying, and Tendering for, a Light Microscope 769 2 Troubleshooting Poor Image Quality 773 3 The Michel-Lévy Interference Colour Chart 775 4 Cleaning and Maintenance of the Light Microscope 779 5 Selected Suppliers 783 6 Historical Background 787 7 Timeline of Key Events 791 Index 799 … (more)
- Edition:
- 1st
- Publisher Details:
- Hoboken, New Jersey : John Wiley & Sons, Inc
- Publication Date:
- 2019
- Extent:
- 1 online resource
- Subjects:
- 570.282
Microscopy - Languages:
- English
- ISBNs:
- 9781118696811
- Related ISBNs:
- 9781118696743
- Notes:
- Note: Includes bibliographical references and index.
Note: Description based on CIP data; resource not viewed. - Access Rights:
- Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
- Access Usage:
- Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.
- View Content:
- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library HMNTS - ELD.DS.408168
- Ingest File:
- 02_485.xml