Enzymes : catalysis, kinetics and mechanisms /: catalysis, kinetics and mechanisms. (2018)

• Record Type: Book
• Further Information: Note: N.S. Punekar.
• Authors: Punekar, N. S
• Contents: Part I: Enzyme catalysis -- a perspective -- Enzymes -Their place in Biology -- Enzymes -- Historical aspects -- Exploiting enzymes – Technology -- On enzyme nomenclature and classification -- Hallmarks of an enzyme catalyst -- Origins of enzyme catalytic power -- Origins of enzyme catalytic power -- Which enzyme uses what trick? Some remarks -- Structure and catalysis: Conformational flexibility and protein motion -- Part II: Enzyme kinetic practice and measurements -- Chemical kinetics – Fundamentals -- Concepts of equilibrium and steady-state -- ES complex and pre-steady-state kinetics -- Principles of enzyme assays -- Good kinetic practices -- Quantification of catalysis and measures of enzyme purity -- Henri-Michaelis-Menten equation -- More complex rate expressions -- Enzyme kinetic data -- Collection and analysis -- Part III: Elucidation of Kinetic mechanisms -- Approaches to kinetic mechanism – Overview -- Analysis of initial velocity patterns -- Enzyme inhibition analyses -- Irreversible inhibitions -- Reversible inhibitions -- Alternate substrate (product) interactions -- pH Studies with enzymes -- Isotopes in enzymology -- Isotope exchanges at equilibrium -- Isotope effects in enzymology -- From kinetic data to mechanism and back -- Part IV: Chemical mechanisms and catalysis -- Chemical reactivity and molecular interactions -- Acid-base chemistry and catalysis -- Nucleophilic catalysis and covalent reaction intermediates -- Phosphoryl group chemistry and importance of ATP -- Enzymatic oxidation-reduction reactions -- Carboxylations and decarboxylations -- Electrophilic catalysis and amino acid transformations -- Integrating kinetic and chemical mechanisms – A synthesis -- Part V: Frontiers in enzymology -- Regulation of enzyme activity -- In vitro versus in vivo -- Concept and consequences -- Future of enzymology – An appraisal -- Closure -- Whither Enzymology.
• Publisher Details: Singapore : Springer
• Publication Date: 2018
• Extent: 1 online resource (xxv, 562 pages), illustrations (some color)
• Subjects: 572/.7; Enzymes; Enzymology; Enzymes; Enzymology; Enzymology; Protein-Ligand Interactions; Biomedical Engineering/Biotechnology; Applied Microbiology; Protein Structure; Electronic books
• Languages: English
• ISBNs: 9789811307850; 9811307857
• Related ISBNs: 9789811307843; 9811307849
• Notes: Note: Includes bibliographical references.; Note: Online resource; title from PDF title page (SpringerLink, viewed November 19, 2018).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.