Advancing development of synthetic gene regulators : with the power of high-throughput sequencing in chemical biology /: with the power of high-throughput sequencing in chemical biology. ([2018])

• Record Type: Book
• Further Information: Note: Anandhakumar Chandran.
• Authors: Chandran, Anandhakumar
• Contents: Supervisor's Foreword; Part of this Thesis have been Published in the Following Journal Articles:Chandran A, Syed J, Li Y, Sato S, Bando T, Sugiyama H (2016) "Genome-Wide Assessment of the Binding Effects of Artificial Transcriptional Activators by High-Throughput Sequencing." Chembiochem 17:1905-1910. doi: 10.1002/cbic.201600274. Chandran A, Syed J, Taylor RD, Kashiwazaki G, Sato S, Hashiya K, Bando T, Sugiyama H (2016) "Deciphering the genomic targets of alkylating polyamide conjugates using high-throughput sequencin; Acknowledgements; Contents. 1 Overview of Next-Generation Sequencing Technologies and Its Application in Chemical BiologyAbstract; 1.1 General Introduction: Next Generation Sequencing (NGS) Principles and Platforms; 1.1.1 Sequencing by Synthesis (SBS); 1.1.1.1 Illumina; 1.1.1.2 Roche 454; 1.1.1.3 Ion Torrent; 1.1.2 Single-Molecule Sequencing (SMS); 1.1.2.1 Helicos; 1.1.2.2 Pacific Biosciences (PacBio); 1.1.3 Sequencing by Ligation (SBL); 1.1.3.1 Polonator and SOLiD (Support Oligonucleotide Ligation Detection); 1.2 Applications of NGS in Chemical Biology; 1.2.1 Genome-Wide Localization of Non-B DNA Using Small Molecules. 1.2.2 Decoding of DNA Base Modification in Single Molecule Level1.2.2.1 Studies Targets Epigenetically Modified Bases; 1.2.2.2 DNA Strand Breakage and Damaged Bases; 1.2.3 Aptamer Selection Using Massively Parallel Sequencing; 1.3 Development of N-Methylpyrrole (P)-N-Methylimidazole (I) Polyamides (PIP) and Its Conjugates; 1.4 PIP Based Gene Regulation; 1.5 Utilization of NGS in PIP Based Small Molecule Studies; 1.5.1 Guiding the Design and Screening of Small Molecule Using NGS; 1.5.2 Analysis of Gene Expression Induced by PIP Based Small Molecules; 1.5.2.1 Transcriptome Studies. 2.3.1.1 General Scheme for Synthesis of Biotin-Conjugated Polyamides2.3.2 Oligomer Sequences Used in Bind-n-Seq Method; 2.3.3 Bind-n-Seq; 2.3.4 Surface Plasmon Resonance (SPR); 2.3.4.1 General Scheme for Synthesis of Polyamides Used for SPR Analysis; 2.3.4.2 SPR Analysis; References; 3 Genome-Wide Assessment of the Binding Effects of Artificial Transcriptional Activators by Utilizing the Power of High-Throughput Sequencing; Abstract; 3.1 Introduction; 3.2 Results and Discussion; 3.2.1 Revealing the Unique Gene Set Activation Mechanism of PIP Conjugates in the Human Genome.
• Publisher Details: Singapore : Springer
• Publication Date: 2018
• Extent: 1 online resource (xv, 114 pages), illustrations (some color)
• Subjects: 572.8/65; Genetic regulation; SCIENCE -- Life Sciences -- Biochemistry; Genetic regulation; Chemistry; Biotechnology; Gene Therapy; Bioorganic Chemistry; Gene Expression Regulation; Electronic books; Electronic book
• Languages: English
• ISBNs: 9789811065477; 9811065470
• Related ISBNs: 9789811065460; 9811065462
• Notes: Note: Includes bibliographical references.; Note: Online resource; title from PDF title page (SpringerLink, viewed October 3, 2017).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.