Development of a fully integrated sample-in-answer-out system for automatic genetic analysis. (2018)

• Record Type: Book
• Further Information: Note: Bin Zhuang.
• Authors: Zhuang, Bin
• Contents: Chapter 1: Introduction; 1.1 Genetic Analysis; 1.1.1 DNA Extraction; 1.1.2 DNA Amplification; 1.1.3 DNA Detection; 1.1.3.1 Nucleic Acid Electrophoresis; 1.1.3.2 DNA Microarray; 1.1.3.3 Quantitative PCR; 1.1.3.4 Gene Sequencing; 1.2 Microfluidic; 1.2.1 Silicon-Based Materials; 1.2.2 Paper-Based Materials; 1.2.3 Polymers-Based Material; 1.3 Microfluidic for Integrated Gene Detection; 1.3.1 Micrfluidic for DNA Extraction; 1.3.2 On-Chip Amplification; 1.3.3 Capillary Electrophoresis Chip; 1.3.4 Integrated Genetic Analysis System1.4 The Purpose and Significance of This Study; 1.5 Main Lines of Each Chapter; References; Chapter 2: Construction of a General Platform for Capillary Electrophoresis; 2.1 Chapter Introduction; 2.2 Construction of Capillary Electrophoresis Instrument; 2.2.1 Optical System; 2.2.1.1 DNA Fluorescence Detection; 2.2.1.2 Optical Design; 2.2.1.3 Mechanical Design and Adjustment; 2.2.2 Circuit Board Sets; 2.2.2.1 Scanning Stage Control; 2.2.2.2 High-Voltage Module; 2.2.2.3 Signal Processing Module; 2.2.3 Chip Loading Stage; 2.2.4 Industrial Design; 2.2.5 Software and Operation Process2.2.5.1 Start; 2.2.5.2 Scan Chip; 2.2.5.3 Find Channels; 2.2.5.4 Operation; 2.2.5.5 Results; 2.2.5.6 Exit; 2.3 Capillary Electrophoresis Chip; 2.3.1 Design of Glass Capillary Electrophoresis Chip; 2.3.2 Manufacture of Capillary Electrophoresis Chip; 2.4 Instrument Verification; 2.4.1 PCR Amplification; 2.4.2 Pre-treatment of Glass Electrophoresis Chip; 2.4.3 Test Results; 2.5 Chapter Summary; References; Chapter 3: Integrated Module for Automated DNA Extraction and Amplification; 3.1 Chapter Introduction; 3.2 Integrated Chip for DNA Extraction and Amplification3.2.1 On-Chip Valve Structure; 3.2.2 Manufacture of DNA Extraction and Amplification Chip; 3.2.3 Verification on the On-Chip Valve Structure; 3.3 DNA Extraction and Amplification Equipment; 3.4 Calibration of the Integrated Sample Preparation System; 3.4.1 Calibration of PCR Temperature Curve; 3.4.2 The Automated Extraction and Amplification Process; 3.5 Verification of Automated DNA Extraction and Amplification System; 3.6 Screening of Congenital Hearing Loss Mutations Using Automated Sample Preparation System; 3.6.1 Experimental Setup; 3.6.2 Experimental Results3.7 Chapter Summary; References; Chapter 4: Fully Integrated Genetic Analysis System; 4.1 Chapter Introduction; 4.2 Design and Construction of the Fully Integrated Interface; 4.2.1 Deign of the Interface; 4.2.2 Design of Sample Electrode; 4.2.3 Verification of the S Electrode Design; 4.3 Upgrade of the Fully Integrated Chip; 4.4 Upgrade of the Integrated Instrument; 4.4.1 Upgrade of Instrument Design and Structure; 4.4.2 Upgrade of the Automated Operations; 4.5 Verification of the Fully Integrated System; 4.5.1 Verification of the Integrated Interface.
• Publisher Details: Singapore : Springer
• Publication Date: 2018
• Extent: 1 online resource
• Subjects: 572.8; DNA -- Analysis; Microfluidics; SCIENCE -- Life Sciences -- Biochemistry; DNA -- Analysis; Microfluidics; Nucleic Acid Chemistry; DNA -- analysis; Electronic books
• Languages: English
• ISBNs: 9789811047534; 9811047537
• Related ISBNs: 9811047529; 9789811047527
• Notes: Note: Includes bibliographical references at the end of each chapters.
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.