Single cell analysis : contemporary research and clinical applications /: contemporary research and clinical applications. (2017)

• Record Type: Book
• Further Information: Note: J. Paul Robinson, Andrea Cossarizza, editors.
• Editors: Robinson, J. Paul; Cossarizza, Andrea, 1958-
• Contents: Preface; Acknowledgements; Contents; Contributors; 1 Microenvironment Cytometry; Abstract; 1 Microcommunities and Microenvironments; 1.1 Introduction; 1.1.1 Boundaries and Niches; 1.1.2 Tumor Microenvironment [TME]; 2 Plasticity of Cellular Communities; 2.1 Landscapes and Attractor States; 2.2 Cell Transitions and Interactions: The Glycome; 2.3 Cellular Interactions in a Microcommunity: Transitions in NCAM Polysialylation; 3 Chromosomal Instability (CIN) and the Microenvironment; 3.1 Cytometric Analysis of Chromosomal Instability; 3.2 Mitotic Bypass; 3.3 Mitotic Bypass and the p53 Network. 4 In Vitro Microenvironment Models and Hollow Fiber Technology4.1 3D Models: Multicellular Tumor Spheroids; 4.2 Encapsulation Systems; 4.2.1 Microencapsulation and Macroencapsulation; 4.2.2 HF Bioreactor (HFB) Cell-Culture Systems; 4.2.3 HF Microenvironment Control; 4.2.4 HF Implant Assay; 4.2.5 HFA Cytometry; 5 Microenvironment and Hypoxia; 5.1 Modeling the Hypoxic Microenvironment; 5.2 Stromal-Tumor Hypoxia; 5.3 Hypoxia Detection; 5.4 Cellular Responses to the Hypoxic Tumor Environment; 6 Targeting Hypoxia; 6.1 The Therapeutic Paradigm; 6.2 Hypoxia-Activated Prodrugs: HAPs. 6.3 Unidirectional HAPs (uHAPs) and Multilevel Targeting7 The Continuing Challenge; Acknowledgements and Declarations; References; 2 Rare Cells: Focus on Detection and Clinical Relevance; Abstract; 1 Introduction; 2 Ag-Specific T Cells; 3 Invariant Natural Killer T (iNKT) Cells; 4 Circulating Endothelial Cells and Their Precursors; 5 Circulating Tumor Cells; 6 Analysis of Rare Events by Flow Cytometry: The First Step; 6.1 Enrichment and Choice of Markers; 6.2 Number of Acquired Events; 6.3 Sample Concentration and Flow Rate; 6.4 Thresholds, Gating, and DUMP Channel; 6.5 Data Analysis. 4 Cytomics of Oxidative Stress: Probes and ProblemsAbstract; 1 Introduction to Reactive Oxygen (ROS) and Nitrogen (RNS) Species; 2 The Physiological Side of ROS and NOS; 2.1 Sources of ROS and RNS; 2.2 ROS in Phagocytosis; 2.3 ROS and RNS in Cellular Signaling; 3 Oxidative Stress: Definition, Causes, and Consequences; 3.1 Definition of Oxidative Stress; 3.2 Causes of Oxidative Stress; 3.3 Consequences of Oxidative Stress; 3.3.1 Protein Damage; 3.3.2 Lipid Peroxidation; 3.3.3 Oxidative Lesions to DNA; 4 Strategies and Reagents for Cytomic Analysis of Oxidative Stress.
• Publisher Details: Singapore : Springer
• Publication Date: 2017
• Extent: 1 online resource
• Subjects: 616.07/582; 620; Flow cytometry; TECHNOLOGY & ENGINEERING -- Engineering (General); TECHNOLOGY & ENGINEERING -- Reference; Flow cytometry; Engineering; Biomedical Engineering; Immunology; Biological and Medical Physics, Biophysics; Cancer Research; Cell Biology; Flow Cytometry; Electronic books
• Languages: English
• ISBNs: 9789811044991; 9811044996
• Related ISBNs: 9789811044984; 9811044988
• Notes: Note: Online resource; title from PDF title page (SpringerLink, viewed May 1, 2017).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.