Handbook of vascular biology techniques. (2015)

• Record Type: Book
• Further Information: Note: Mark Slevin, Garry McDowell, editors.
• Editors: Slevin, Mark; McDowell, Garry
• Contents: Preface -- Section 1 In vitro techniques -- In vitro angiogenesis assay -- endothelial migration, proliferation, and tube formation -- Endothelial cell tube formation on basement membrane to study cancer neoangiogenesis -- Induction of hypoxia in vascular endothelial cell culture -- Evaluating In Vitro Angiogenesis using Live Cell Imaging -- Isolation of Endothelial Progenitor Cells (EPCs) -- Culture and Maintenance of Human Embryonic Stem Cells: A Potential Source for Vasculargenesis -- Assessment of vascular function and contractility, ex vivo -- Measurement of AÝ uptake by cerebrovascular smooth muscle cells -- Measurement of Intracellular Ca2+ in Human Endothelial cells -- Section 2 In vivo and ex vivo manipulations -- Evaluation of angiogenesis and arteriogenesis in a mouse model of prolonged cerebral hypoperfusion -- Sponge implant model of inflammatory angiogenesis -- The chick embryo chorioallantoic membrane assay -- Dorsal air sac assay -- Scanning Electron Microscopy of Blood Vascular Corrosion Casts (SEM on VCC) in mammals -- Hypoxia-induced retinal angiogenesis in adult zebrafish -- Angiogenesis in regenerating adult zebrafish tail fin -- Methods for studying developmental angiogenesis in zebrafish -- Isolation and Expansion of Brain Microvascular Endothelial Cells -- Section 3 Imaging and histological analysis -- Adipose angiogenesis -- Assessing tumor angiogenesis in histological samples -- Whole-mount immunostaining methods to study the blood and lymphatic vasculature in the embryonic mouse skin and adult mouse cornea -- Computed Tomography Angiography: Fundamental techniques and data interpretation -- Magnetic Resonance Angiography: Fundamental Techniques and Data Interpretation -- Single-Photon Emission Tomography of the brain in vascular pathology -- Section 4 Miscellaneous novel techniques in vascular biology -- Enhancing Endothelialisation of Artificial/Engineered Blood Vessels Using Structural Cues -- Preparation of liposomes with dual fluorophores to follow real-time content release in vivo -- Vascular Flow Modelling using Computational Fluid Dynamics -- Reverse Transcription Real-Time PCR Protocol for gene expression analyses -- Oscillations, Feedback and Bifurcations in Mathematical Models of Angiogenesis and Haematopoiesis -- Genomic microarray analysis -- Selection of appropriate housekeeping genes for quality control -- Endothelial transcriptomic analysis -- Protocol for Multiplex Amplicon Sequencing using Barcoded Primers -- Flow cytometry Enumeration of Hematopoietic & Progenitor Stem Cells: Identification and Quantification -- A Scheme for the Development and Validation of Enzyme Linked Immunosorbent Assays (ELISA) for Measurement of Angiogenic Biomarkers in Human Blood -- Analysis of Phosphorylated Protein Kinases in Endothelial cells by Flow Cytometry.
• Publisher Details: Dordrecht : Springer
• Publication Date: 2015
• Extent: 1 online resource (xii, 477 pages), illustrations (some color)
• Subjects: 612.1/3; Blood-vessels -- Handbooks, manuals, etc; Blood-vessels -- Physiology -- Handbooks, manuals, etc; MEDICAL -- Physiology; SCIENCE -- Life Sciences -- Human Anatomy & Physiology; Blood-vessels; Blood-vessels -- Physiology; Biomedicine; Cancer Research; Biological Techniques; Laboratory Medicine; Blood Vessels -- physiology; Electronic books; Handbooks and manuals
• Languages: English
• ISBNs: 9789401797160; 9401797161
• Related ISBNs: 9789401797153; 9401797153; 9789401797153
• Notes: Note: Includes bibliographical references at the end of each chapters and index.; Note: Online resource; title from PDF title page (SpringerLink, viewed April 1, 2015).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.