Protein NMR : modern techniques and biomedical applications /: modern techniques and biomedical applications. (2015)

• Record Type: Book
• Further Information: Note: Lawrence Berliner, editor.
• Editors: Berliner, Lawrence J
• Contents: 4.7 Completing Side-Chain 13C/1H Assignments Using 13C HCCH-COSY/TOCSY4.8 Triple Resonance Methods with Deuteration; 4.9 Triple Resonance Methods for Proteins Above 40 kD; 5 The Sequential Assignment Method; 5.1 2D and 3D Experiments for 15N-Labelled Proteins; 5.2 Stage 1: Spin System Identification from 3D TOCSY-HSQC and 3D HNHA; 5.3 Stage 2: Sequence-Specific Assignment from 3D NOESY-HSQC and HSQC-NOESY-HSQC; 6 Conclusion; References; Mapping Out Protein Hydration Dynamics by Overhauser Dynamic Nuclear Polarization; 1 Introduction; 1.1 Biophysical Tools to Study Protein Hydration. 1.2 Introduction to ODNP2 Methodology; 2.1 Theory of ODNP in Solution; 2.2 Experimental Quantification of ODNP in Solution; 3 Application to Protein Folding, Protein Aggregation, Membrane Protein Topology, and Membrane-Protein Interaction; 4 Opportunities of Overhauser DNP for Studying Protein Hydration; References; Relaxation Dispersion NMR Spectroscopy; 1 Introduction; 1.1 History-Development of Relaxation Dispersion NMR; 1.2 Theoretical Aspects of Chemical Exchange; 2 Relaxation Dispersion Experiments; 2.1 Nitrogen-Based Relaxation Dispersion Experiments. 4.4 Structure Determination in Membrane-Mimetics4.5 Dynamics; 5 Outlook; References; Line Narrowing in Oriented-Sample NMR of Membrane Proteins; 1 Introduction; 1.1 Why Study Membrane Proteins in Their Native-like Bilayer Environment?; 1.2 Solid-State NMR Methods for Structure Determination of Membrane Proteins; 1.3 Structural Information from Solid-State NMR of Macroscopically Aligned Samples; 2 Requirements for High-Resolution Oriented-Sample NMR Spectra of Membrane Proteins; 2.1 Effects of Uniaxial Rotational Diffusion on Solid-State NMR Spectra of Membrane Proteins.
• Publisher Details: New York : Springer
• Publication Date: 2015
• Extent: 1 online resource (x, 185 pages), illustrations (some color)
• Subjects: 572/.636; Proteins -- Analysis; Nuclear magnetic resonance; SCIENCE -- Life Sciences -- Biochemistry; Nuclear magnetic resonance; Proteins -- Analysis; Biomedicine; Molecular Medicine; Nuclear Magnetic Resonance, Biomolecular; Electronic books; Electronic books
• Languages: English
• ISBNs: 9781489976215; 1489976213; 1489976205; 9781489976208
• Related ISBNs: 9781489976208
• Notes: Note: Online resource; title from PDF title page (SpringerLink, viewed September 4, 2015).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.