Nanotechnology for Nucleic Acid Delivery : Methods and Protocols /: Methods and Protocols. (2019)

• Record Type: Book
• Further Information: Note: Manfred Ogris, Haider Sami.
• Editors: Ogris, Manfred; Sami, Haider
• Contents: 1. Sequence-Defined Cationic Lipo-Oligomers Containing Unsaturated Fatty Acids for Transfection Sören Reinhard and Ernst Wagner 2. Synthesis of Bioreducible Polycations with Controlled Topologies Ye-Zi You, Jun-Jie Yan, Fei Yu, Zhi-Qiang Yu, and David Oupický 3. Histidine-Rich Cationic Cell Penetrating Peptides for Plasmid DNA and siRNA Delivery Antoine Kichler, A. James Mason, Arnaud Marquette, and Burkhard Bechinger 4. Covalent Fluorophore Labeling of Oligonucleotides and Generation of Other Oligonucleotide Bioconjugates Cornelia Lorenzer and Johannes Winkler 5. Synthetic Oligonucleotides in SPECT/CT In Vivo Imaging: Chemical Modifications, In111 Complex Formation, Incorporation into Drug Delivery Systems Maxim Antopolsky 6. Synthesis of Polyethylenimine-Based Nanocarriers for Systemic Tumor Targeting of Nucleic Acids Wolfgang Rödl, Alexander Taschauer, David Schaffert, Ernst Wagner, and Manfred Ogris 7. Cationic Photopolymerized Polydiacetylenic (PDA) Micelles for siRNA Delivery Manon Ripoll, Patrick Neuberg, Jean-Serge Remy, and Antoine Kichler 8. Lipids for Nucleic Acid Delivery: Cationic or Neutral Lipoplexes, Synthesis, and Particle Formation Michel Bessodes, Helene Dhotel, and Nathalie Mignet 9. Preparation, Characterization, and In Vitro Evaluation of Lipidoid-Polymer Hybrid Nanoparticles for siRNA Delivery to the Cytosol Kaushik Thanki, Xianghui Zeng, and Camilla Foged 10. Layer-By-Layer Assembled Nanoparticles for siRNA Delivery Michaela Guter and Miriam Breunig 11. Layer-By-Layer Film Engineering for Sequential Gene Delivery Lingxiao Xie, Yi Zou, Sean Carroll, Maria Muniz, and Guangzhao Mao 12. Surface- and Hydrogel-Mediated Delivery of Nucleic Acid Nanoparticles Angela K. Pannier, Tyler Kozisek, and Tatiana Segura 13. In Situ AFM Analysis Investigating Disassembly of DNA Nanoparticles and Nano-Films Yi Zou, Lei Wan, Jenifer Blacklock, Lingxiao Xie, Sean Carroll, David Oupicky, and Guangzhao Mao 14. Lyophilization of Synthetic Gene Carriers Julia Christina Kasper, Sarah Hedtrich, and Wolfgang Friess 15. Firefly Luciferase-Based Reporter Gene Assay for Investigating Nanoparticle-Mediated Nucleic Acid Delivery Katharina Müller, Manfred Ogris, and Haider Sami 16. Enhancing Nucleic Acid Delivery with Ultrasound and Microbubbles Heleen Dewitte, Silke Roovers, Stefaan C. De Smedt, and Ine Lentacker 17. Magnetic and Acoustically Active Microbubbles Loaded with Nucleic Acids for Gene Delivery Dialechti Vlaskou, Olga Mykhaylyk, and Christian Plank 18. Lactate Dehydrogenase Assay for Assessment of Polycation Cytotoxicity Ladan Parhamifar, Helene Andersen, and S. Moein Moghimi 19. Combined Fluorimetric Caspase 3/7 Assay and Bradford Protein Determination for Assessment of Polycation-Mediated Cytotoxicity Anna K. Larsen, Arnaldur Hall, Henrik Lundsgart, and S. Moein Moghimi 20. Determination of Polycation-Mediated Perturbation of Mitochondrial Respiration in Intact Cells by High-Resolution Respirometry (Oxygraph-2k, OROBOROS) Arnaldur Hall and S. Moein Moghimi 21. Evaluating the Regulation of Cytokine Levels after siRNA Treatment in Antigen-Specific Target Cell Populations via Intracellular Staining Rima Kandil, Daniel Feldmann, Yuran Xie, and Olivia M. Merkel 22. Anti-PEG IgM Production via a PEGylated Nano-Carrier System for Nucleic Acid Delivery Amr S. Abu Lila and Tatsuhiro Ishida 23. Near-Infrared Optical Imaging of Nucleic Acid Nanocarriers In Vivo Claire Rome, Julien Gravier, Marie Morille, Gilles Divita, Anne-Laure Bolcato-Bellemin, Véronique Josserand, and Jean-Luc Coll 24. Flow Cytometry-Based Cell Type-Specific Assessment of Target Regulation by Pulmonary siRNA Delivery Olivia M. Merkel, Leigh M. Marsh, Holger Garn, and Thomas Kissel 25. Microbubbles for Nucleic Acid Delivery in Liver Using Mild Sonoporation Nathalie Mignet, Corinne Marie, Anthony Delalande, Simona Manta, Michel-Francis Bureau, Gilles Renault, Daniel Scherman, and Chantal Pichon 26. Lipopeptide Delivery of siRNA to the Central Nervous System Mark D. Zabel, Luke Mollnow, and Heather Bender.
• Edition: Second edition
• Publisher Details: New York : Humana Press
• Publication Date: 2019
• Copyright Date: 2019
• Extent: 1 online resource (406 pages)
• Subjects: Medicine; Technology & Engineering -- Nanotechnology & MEMS; Science -- Biotechnology; Medical -- Pharmacology; Nanotechnology; Biotechnology; Pharmacology; Human genetics; Nanotechnology; Biotechnology; Toxicology; Medical -- Genetics; Medical genetics
• Languages: English
• ISBNs: 9781493990924
• Related ISBNs: 9781493990917
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.