Intracellular Lipid Transport : Methods and Protocols /: Methods and Protocols. (2019)

• Record Type: Book
• Further Information: Note: Guillaume Drin.
• Editors: Drin, Guillaume
• Contents: 1. Advanced quantitative measure of sterol movements in mammal cells (example of STARD protein). 2. Use of new probes to detect sphingolipids 3. How to measure ceramide transport. 4. Update on the strategy to follow the movement of DAG and PA lipids. 5. TIRF microscopy to measure lipid movement in and out the PM with fluorescent-tagged probe 6. BRET & FRET approaches to measure intracellular lipid transport. 7. Measure of dynamics of native lipid species (caged-lipids) in cells. 8. Cell fractionation and lipidomics to measure lipid movements in organelles. 9. PS and PE transport at ER-mitochondria contact sites and inside mitochondria 10. Lipid transport and endosomal-lysosomal compartment. 11. Measurement of neutral lipid and sterol accumulation in lipid droplets. 12. Lipid transport in plants (ER-chloroplast contact sites). 13. Measure of glycolipids transport. 14. Identification of putative lipid-transporter by sequence analysis. 15. Identification of lipid transporter and their ligand by purification affinity and denaturating mass spectrometry 16. Use of click-chemistry to identify novel lipid transporters and their ligands. 17. In vitro real-time measure of lipid exchange by ORP/Osh protein - Guillaume Drin et al. 18. Measure of lipid transport by liposome separation approaches 19. In silico examination of membrane binding and lipid release activity of an LTP 20. Advanced approaches to measure lipid scramblase/flippase activity 21. Additionnal : mathematical approaches for modeling lipid transport in cells.
• Publisher Details: New York : Humana Press
• Publication Date: 2019
• Copyright Date: 2019
• Extent: 1 online resource (345 pages)
• Subjects: Life sciences; Science -- Life Sciences -- Cytology; Cellular biology (cytology); Lipids; Cell membranes; Science -- Life Sciences -- Biochemistry; Lipids
• Languages: English
• ISBNs: 9781493991365
• Related ISBNs: 9781493991358
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.