Recombinant Protein Production in Yeast. (2019)

• Record Type: Book
• Further Information: Note: Brigitte Gasser, Diethard Mattanovich.
• Editors: Gasser, Brigitte; Mattanovich, Diethard
• Contents: 1. Established and Upcoming Yeast Expression Systems Burcu Gündüz Ergün, Damla Hüccetoğulları, Sibel Öztürk, Eda Çelik, and Pınar Çalık 2.Detection and Elimination of Cellular Bottlenecks in Protein Producing Yeasts Richard J. Zahrl, Brigitte Gasser, Diethard Mattanovich, and Pau Ferrer 3.Pichia pastoris Promoters Aysun Özçelik, Semiramis Yılmaz, and Mehmet Inan 4. Selection of the Optimal Yeast Host for the Synthesis Recombinant Enzymes Felix Bischoff, Martin Giersberg, Falko Matthes, Tobias Schwalenberg, Sebastian Worch, and Gotthard Kunze 5. Screening and Selection of Production Strains: Secretory Protein Expression and Analysis in Hansenula polymorpha Su Jin Yoo, Hye Yun Moon, and Hyun Ah Kang 6. Selection of Heterologous Protein Producing Strains in Yarrowia lipolytica Paul Soudier, Macarena Larroude, Ewelina Celinska, Tristan Rossignol, and Jean-Marc Nicaud 7. High-throughput Screening and Selection of Pichia pastoris Strains Roland Weis 8. Analyzing Recombinant Protein Production in Pichia pastoris with Targeted Proteomics Roslyn M. Bill, Annegret Ulke-Lemée, Stephanie P. Cartwright, Rena Far, Jay Kim, and Justin A. MacDonald 9. CRISPR/Cas9-mediated Homology Directed Genome Editing in Pichia pastori Thomas Gassler, Lina Heistinger, Diethard Mattanovich, Brigitte Gasser, and Roland Prielhofer 10. Tailoring N-glycan Biosynthesis for Production of Therapeutic Proteins in Saccharomyces cerevisiae Mari A. Piirainen, Heidi Salminen and Alexander D. Frey 11. Improving the Production of Cofactor Containing Proteins: Production of Human Hemoglobin in Yeast Olena P. Ishchuk, José L. Martínez, and Dina Petranovic 12. Membrane Protein Production in Yeast: Modification of Yeast Membranes for Human Membrane Protein Production Anita Emmerstorfer-Augustin, Tamara Wriessnegger, Melanie Hirz, Guenther Zellnig, and Harald Pichler 13. Yeast Surface Display and Cell Sorting of Antigen-binding Fc Fragments Flávio Sádio, Gerhard Stadlmayr, Katharina Stadlbauer, Florian Rüker, and Gordana Wozniak-Knopp 14. Guidelines for Small-scale Production and Purification of Hepatitis B surface Antigen Virus-like Particles from Recombinant Pichia pastoris Maria Zahid and Ursula Rinas 15. Efficient Development of a Mixed Feed Process for Pichia pastoris David Johannes Wurm and Oliver Spadiut 16. Primary Recovery of Yeast Culture Supernatant for Recombinant Protein Purification Michael M. Maurer and Harald Schillinger 17. Purification of Recombinant Glyco-proteins from Pichia pastoris Culture Supernatants David Johannes Wurm and Oliver Spadiut 18. LC-MS analysis of (glyco)-proteins of Pichia pastoris Clemens Grünwald-Gruber and Friedrich Altmann 19. Assessing the Quality of Recombinant Products Made in Yeast Karola Vorauer-Uhl and Gabriele Lhota 20. Quantification of Recombinant Products In Yeast Karola Vorauer-Uhl and Gabriele Lhota.
• Publisher Details: New York : Humana Press
• Publication Date: 2019
• Copyright Date: 2019
• Extent: 1 online resource (431 pages)
• Subjects: Chemistry; Science -- Life Sciences -- Biochemistry; Proteins; Biotechnology; Biochemistry; Science -- Biotechnology; Biotechnology
• Languages: English
• ISBNs: 9781493990245
• Related ISBNs: 9781493990238
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.