Vertebrate Embryogenesis : Embryological, Cellular, and Genetic Methods /: Embryological, Cellular, and Genetic Methods. (2019)

• Record Type: Book
• Further Information: Note: Francisco J. Pelegri.
• Editors: Pelegri, Francisco J
• Contents: 1. Culture and Host Transfer of Xenopus Oocytes for Maternal mRNA Depletion and Genome Editing Experiments Douglas W. Houston 2. Immunofluorescence of Microtubule Assemblies in Amphibian Oocytes and Early Embryos Thao Nguyen, Timothy J. Mitchison, and Martin Wühr 3. Assaying NanoLuc Luciferase Activity from mRNA-Injected Xenopus Embryos Michael D. Sheets 4. Reconstitution of Intracellular Calcium Signaling in Xenopus Egg Extracts Alexander A. Tokmakov and Ken-Ichi Sato 5. Membrane Microdomains as Platform to Study Membrane-Associated Events during Oogenesis, Meiotic Maturation, and Fertilization in Xenopus laevis Ken-ichi Sato and Alexander A. Tokmakov 6. Embryological and Genetic Manipulation of Chick Development Laura S. Gammill, Bridget Jacques-Fricke, and Julaine Roffers-Agarwal 7. Identifying Protein-DNA and Protein-Protein Interactions in Avian Embryos Ana Paula Azambuja and Marcos Simoes-Costa 8. Experimental Manipulation of Ploidy in Zebrafish Embryos and its Application in Genetic Screens Triveni Menon and Sreelaja Nair 9. RNA Tomography for Spatially Resolved Transcriptomics (Tomo-Seq) Karoline Holler and Jan Philipp Junker 10. Optogenetic Control of Subcellular Protein Location and Signaling in Vertebrate Embryos Clare E. Buckley 11. Ex Utero Culture and Imaging of Mouse Embryos Sonja Nowotschin, Vidur Garg, Anna Piliszek, and Anna-Katerina Hadjantonakis 12. Detection of Gene and Protein Expression in Mouse Embryos and Tissue Sections Edwina McGlinn, Miriam Holzman, and Jennifer H. Mansfield 13. Reptile Embryology and Regeneration Matthew K. Vickaryous and Emily A.B. Gilbert 14. Manipulation of Developmental Function in Turtles with Notes on Alligators Jacqueline E. Moustakas-Verho, Rebecca McLennan, Jennifer Spengler, Paul M. Kulesa, and Judith A. Cebra-Thomas 15. Methods for Isolating the Balbiani Body/Germ Plasm from Xenopus laevis Oocytes Amanda Butler, Dawn Owens, Mary Lou King, and Tristan Aguero 16. Visualizing the Balbiani Body in Zebrafish Oocytes KathyAnn L. Lee and Florence L. Marlow 17. Isolation of Zebrafish Balbiani Bodies for Proteomic Analysis Allison Jamieson-Lucy and Mary C. Mullins 18. Generation of Xenopus Haploid, Triploid, and Hybrid Embryos Romain Gibeaux and Rebecca Heald 19. Germ Cell Transplantation in Avian Species Young Hyun Park, Young Min Kim, and Jae Yong Han 20. Production of Germ-Line Chimeras in Zebrafish Taiju Saito, Rie Goto, Nicola Rivers, and Etsuro Yamaha 21. A Method for Zebrafish Follicle Transplantation into Recipient Mothers for the Generation of Fertilizable Eggs and Viable Offspring Zsolt Csenki and Ferenc Mueller 22. Optimized Protocol of Zebrafish Somatic Cell Nuclear Transfer (SCNT) Sukumal Prukudom, Kannika Siripattarapravat, William Poulos, and Jose B. Cibelli 23. An Accessible Protocol for the Generation of CRISPR- Cas9 Knockouts Using INDELs in Zebrafish Cara E. Moravec and Francisco J. Pelegri 24. Modeling Embryonic Cleavage Patterns Dmitry Ershov and Nicolas Minc 25. Laboratory Guidelines for Animal Care Marcelo Couto and Charles Cates.
• Edition: Second edition
• Publisher Details: New York : Humana Press
• Publication Date: 2019
• Copyright Date: 2019
• Extent: 1 online resource (434 pages)
• Subjects: Life sciences; Embryology; Laboratory animals; Animal genetics; Science -- Life Sciences -- Zoology -- General; Animal pathology & diseases; Animal reproduction; Science -- Life Sciences -- Biology -- Developmental Biology; Cellular biology (cytology)
• Languages: English
• ISBNs: 9781493990092
• Related ISBNs: 9781493990085
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.